Institut Fresnel, Aix-Marseille Université, CNRS, Ecole Centrale Marseille, Domaine Universitaire de Saint-Jérôme, 13397 Marseille cedex 20, France.
Int J Mol Sci. 2010 Jan 13;11(1):206-221. doi: 10.3390/ijms11010206.
Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution, and how to enhance the faint optical signal emitted per molecule? The focus is set primarily on the widely used technique of fluorescence correlation spectroscopy (FCS), yet the discussion can be extended to other single molecule detection methods.
最近在纳米光子学方面的进展为高效单分子荧光分析的有前途的应用开辟了道路。在这篇综述中，我们讨论了光子方法如何为两个基本问题带来创新的解决方案：如何在高浓度溶液中检测单个分子，以及如何增强每个分子发出的微弱光学信号？重点主要放在广泛使用的荧光相关光谱学（FCS）技术上，但讨论也可以扩展到其他单分子检测方法。
