利用随机扩增多态性DNA（RAPD）技术对食用土当归进行遗传分析。
Genetic analysis of Aralia cordata Thunb by RAPD.
作者信息
Qu Fan, Zhou Jue, Zhou Zhou, Li Huiyu, Burrows Elizabeth
机构信息
School of Medicine, Zhejiang University, 388 Yuhang Tang Road, Hangzhou, Zhejiang 310058, PR China.
出版信息
Afr J Tradit Complement Altern Med. 2008 Oct 25;6(1):17-22.
In the research, genetic analysis of Aralia cordata Thunb. (Araliaceae) was conducted using randomly amplified polymorphism DNA (RAPD). 161 loci were detected with 12 RAPD primers. Percentage of Polymorphic Band (PPB) varied from 34.78% to 63.35%. All the samples were respectively collected from the eight provinces richest in Aralia cordata Thunb resources in China, including Hunan, Yunnan, Zhejiang, Sichuan, Jiangxi, Anhui, Shanxi and Gansu. The results showed that Hunan Province enjoyed the highest level of genetic differentiation and Gansu was the lowest. The total genetic diversity (H(T)) of RAPD, intraspecific genetic diversity (H(S)) and genetic diversity (D(ST)) of the various places was respectively 26.33%, 11.14%, and 49.36%. The differentiation among the species accounted for 98.76% of total genetic diversity (G(ST)). Based on the cluster results of genetic distance, the 8 samples were classified into three groups. It is concluded that Hunan Province enjoyed the highest level of genetic differentiation of Aralia cordata Thunb and Gansu was the lowest, which provides a basis for the taxonomic identification and germplasm resource research of Aralia cordata Thunb in the future.
在该研究中，利用随机扩增多态性DNA（RAPD）技术对食用土当归（五加科）进行了遗传分析。使用12条RAPD引物检测到161个位点。多态性条带百分比（PPB）在34.78%至63.35%之间变化。所有样本分别采集自中国食用土当归资源最丰富的八个省份，包括湖南、云南、浙江、四川、江西、安徽、山西和甘肃。结果表明，湖南省的遗传分化水平最高，甘肃省最低。RAPD的总遗传多样性（H(T)）、种内遗传多样性（H(S)）以及各地的遗传多样性（D(ST)）分别为26.33%、11.14%和49.36%。物种间的分化占总遗传多样性（G(ST)）的98.76%。基于遗传距离的聚类结果，将8个样本分为三组。研究得出结论，湖南省食用土当归的遗传分化水平最高，甘肃省最低，这为今后食用土当归的分类鉴定和种质资源研究提供了依据。
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