Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Foundation for Revitalization of Local Health Traditions (FRLHT), via Yelhanka, Bangalore 560106, India.
Evid Based Complement Alternat Med. 2011;2011:868720. doi: 10.1093/ecam/neq023. Epub 2011 Feb 20.
Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique. Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.
维达哩是一种阿育吠陀草药，用作壮阳药和通乳剂，也用于制备恰瓦那普拉萨。Ipomoea mauritiana Jacq.（旋花科）、Pueraria tuberosa（Roxb. ex Willd.）DC（豆科）、Adenia hondala（Gaertn.）de Wilde（西番莲科）和Cycas circinalis L.（苏铁科）的块茎均以维达哩的名义进行交易，这造成了阿育吠陀原料药的植物学真实性问题。已经开发出基于 DNA 的标记物来区分Ipomoea mauritiana与其他维达哩候选物。使用随机扩增多态性 DNA (RAPD)技术鉴定了一种特定于Ipomoea mauritiana的假定 600-bp 多态性序列。此外，还从独特的 RAPD 扩增子设计了序列特征扩增区域 (SCAR)引物 (IM1F 和 IM1R)。SCAR 引物在真实的Ipomoea mauritiana中产生了特异性的 323-bp 扩增子，而在相关物种中则没有。
