Department of Alveolar Surgery, Implantology and Radiology, Dental School of the Aristotle University of Thessaloniki, Thessaloniki, Greece.
Int J Oral Maxillofac Implants. 2009 Nov-Dec;24(6):1054-60.
The influence of bone marrow in the osteogenic potential of bone has not been evaluated in the rabbit tibia model. Previous studies employed this model extensively to test the osteogenic capabilities of bone grafts. The primary aim of the present study was to assess the role of bone marrow in the healing of experimental defects in the rabbit tibia model.
Ten New Zealand rabbits were divided into two groups of five each. In the first experimental test group (PoP), the marrow cavity was emptied completely under a 6-mm defect in the rabbit tibia metaphysis; the marrow was replaced by plaster of Paris, and a round piece of lyophilized collagen membrane was placed in contact with the endosteum and rested on the plaster. In the second experimental group (control), similar artificial defects were made, but the marrow was left intact. After 8 weeks, the animals were sacrificed and prepared for histologic and histomorphometric analysis.
There was a statistically significant difference (P = .016) in newly formed bone area between the control group (mean, 68.08% +/- 12.09%) and the PoP group (mean, 54.02% +/- 13.93%). Similarly, there was a statistically significant difference in new bone density (P < .001) between the control group (mean, 95.99% +/- 1.74%) and the PoP group (mean, 75.37% +/- 13.27%).
The current study confirms the significant effect that bone marrow has in bone regeneration and also the true regenerative capabilities of the osseous walls of the defects. The proposed experimental model may be a more reliable method of investigating and comparing the potential of different graft materials and methods.
骨髓对骨骼成骨潜能的影响尚未在兔胫骨模型中进行评估。以前的研究广泛采用该模型来测试骨移植物的成骨能力。本研究的主要目的是评估骨髓在兔胫骨模型实验性缺损愈合中的作用。
10 只新西兰兔分为两组，每组 5 只。在第一实验组（PoP）中，在兔胫骨干骺端 6mm 缺损处完全排空骨髓腔；骨髓用石膏代替，冻干胶原膜的圆形片与骨内膜接触并置于石膏上。在第二实验组（对照组）中，制作类似的人工缺损，但保留骨髓。8 周后，处死动物并进行组织学和组织形态计量学分析。
在对照组（平均 68.08% +/- 12.09%）和 PoP 组（平均 54.02% +/- 13.93%）之间，新形成的骨面积有统计学显著差异（P =.016）。同样，在对照组（平均 95.99% +/- 1.74%）和 PoP 组（平均 75.37% +/- 13.27%）之间，新骨密度也有统计学显著差异（P <.001）。
本研究证实了骨髓对骨再生的显著影响，以及骨缺损骨壁的真实再生能力。所提出的实验模型可能是一种更可靠的方法，用于研究和比较不同移植物材料和方法的潜力。
