Pires Jorge Luís da Silva, de Carvalho Jorge José, Pereira Mario José Dos Santos, Brum Igor da Silva, Nascimento Ana Lucia Rosa, Dos Santos Paulo Gonçalo Pinto, Frigo Lucio, Fischer Ricardo Guimaraes
Department of Periodontology, PhD Candidate in Periodontology, School of Dentistry, State University of Rio de Janeiro, Rio de Janeiro 20551-030, Brazil.
Laboratory of Cell Ultrastructure and Tissue Biology, Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro 20550-900, Brazil.
Materials (Basel). 2021 May 26;14(11):2854. doi: 10.3390/ma14112854.
Bone defects are a challenging clinical situation, and the development of hydroxyapatite-based biomaterials is a prolific research field that, in addition, can be joined by stem cells and growth factors in order to deal with the problem. This study compares the use of synthetic hydroxyapatite and xenograft, used pure or enriched with bone marrow mononuclear fraction for the regeneration of critical size bone defects in rat calvaria through histomorphometric (Masson's staining) and immunohistochemical (anti-VEGF, anti-osteopontin) analysis. Forty young adult male rats were divided into five groups (n = 8). Animals were submitted to critical size bone defects (Ø = 8 mm) in the temporoparietal region. In the control group, there was no biomaterial placement in the critical bone defects; in group 1, it was filled with synthetic hydroxyapatite; in group 2, it was filled with xenograft; in group 3, it was filled with synthetic hydroxyapatite, enriched with bone marrow mononuclear fraction (BMMF), and in group 4 it was filled with xenograft, enriched with BMMF. After eight weeks, all groups were euthanized, and histological section images were captured and analyzed. Data analysis showed that in groups 1, 2, 3 and 4 (received biomaterials and biomaterials plus BMMF), a significant enhancement in new bone matrix formation was observed in relation to the control group. However, BMMF-enriched groups did not differ from hydroxyapatite-based biomaterials-only groups. Therefore, in this experimental model, BMMF did not enhance hydroxyapatite-based biomaterials' potential to induce bone matrix and related mediators.
骨缺损是一种具有挑战性的临床情况，基于羟基磷灰石的生物材料的开发是一个成果丰硕的研究领域，此外，还可以加入干细胞和生长因子来解决这一问题。本研究通过组织形态计量学（Masson染色）和免疫组织化学（抗血管内皮生长因子、抗骨桥蛋白）分析，比较了合成羟基磷灰石和异种移植物（单独使用或富集骨髓单个核细胞部分）在大鼠颅骨临界尺寸骨缺损再生中的应用。将40只成年雄性幼鼠分为五组（n = 8）。对动物的颞顶区进行临界尺寸骨缺损（直径 = 8 mm）处理。对照组在临界骨缺损处未放置生物材料；第1组填充合成羟基磷灰石；第2组填充异种移植物；第3组填充富含骨髓单个核细胞部分（BMMF）的合成羟基磷灰石；第4组填充富含BMMF的异种移植物。八周后，对所有组实施安乐死，并采集和分析组织切片图像。数据分析表明，在第1、2、3和4组（接受生物材料和生物材料加BMMF）中，与对照组相比，新骨基质形成有显著增强。然而，富含BMMF的组与仅使用基于羟基磷灰石的生物材料的组没有差异。因此，在该实验模型中，BMMF并未增强基于羟基磷灰石的生物材料诱导骨基质和相关介质的潜力。
