Hass L F, Kappel W K, Miller K B, Engle R L
J Biol Chem. 1978 Jan 10;253(1):77-81.
Previous reports have suggested the possibility of extensive structural homology between human erythrocyte bisphosphoglycerate synthase (glycerate-1,3-P2 leads to glycerate-2,3-P2) and phosphoglycerate mutase (glycerate-3-P in equilibrium glycerate-2-P). This study lends credence to that conjecture through comparative physicochemical investigations involving peptide mapping, circular dichroism, and immunological techniques. The data indicate that despite differences in function, both enzymes apparently manifest a high degree of similarity in primary, secondary, and tertiary structure. Mapping data also indicate that each protein is comprised of two apparently identical subunits.
先前的报告表明，人红细胞二磷酸甘油酸合酶（甘油酸-1,3-P2生成甘油酸-2,3-P2）与磷酸甘油酸变位酶（甘油酸-3-P与甘油酸-2-P处于平衡状态）之间可能存在广泛的结构同源性。本研究通过涉及肽图谱分析、圆二色性和免疫技术的比较物理化学研究，证实了这一推测。数据表明，尽管功能不同，但这两种酶在一级、二级和三级结构上显然表现出高度相似性。肽图谱分析数据还表明，每种蛋白质都由两个明显相同的亚基组成。
