1-乙基-3-甲基咪唑硫酸乙酯/铜催化剂增强葡萄糖化学发光检测：对发光和酶活性的影响。
1-Ethyl-3-methylimidazolium ethylsulfate/copper catalyst for the enhancement of glucose chemiluminescent detection: effects on light emission and enzyme activity.
机构信息
Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1-CNRS 5246 ICBMS, Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France.
出版信息
Anal Chem. 2010 Mar 15;82(6):2401-4. doi: 10.1021/ac9026725.
The effect of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO(4)]) on the copper-catalyzed luminol chemiluminescence (CL) is reported. A drastic light emission enhancement is observed, related to a strong interaction between Cu(2+) and the imidazolium ring. In these conditions, the CL reaction was able to produce light efficiently at pH as low as 6.5 (amplification factor: Intensity(+IL)/Intensity(-IL) = 2900). Interesting effects of [Emim][EtSO(4)] on the enzyme glucose oxidase activity were also evidenced, and advantages were taken from this enhancement to perform sensitive chemiluminescent glucose detection (LOD = 4 microM) at pH 8.0.
报道了离子液体 1-乙基-3-甲基咪唑硫酸乙酯 ([Emim][EtSO(4)]) 对铜催化鲁米诺化学发光 (CL) 的影响。观察到发光强度的剧烈增强，这与 Cu(2+) 和咪唑环之间的强相互作用有关。在这些条件下，CL 反应能够在 pH 低至 6.5 的情况下有效地产生光（放大因子：Intensity(+IL)/Intensity(-IL) = 2900）。还证明了 [Emim][EtSO(4)] 对酶葡萄糖氧化酶活性的有趣影响，并利用这种增强作用在 pH 8.0 下进行了灵敏的化学发光葡萄糖检测 (LOD = 4 microM)。
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