Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
Talanta. 2012 Sep 15;99:643-8. doi: 10.1016/j.talanta.2012.06.061. Epub 2012 Jun 29.
As a peroxidase mimic, cupric oxide nanoparticles were found to enhance the chemiluminescence (CL) of luminol-H(2)O(2) system up to 400 folds. The CL spectra and radical scavengers were conducted to investigate the possible CL enhancement mechanism. It was suggested that the enhanced CL could be attributed to the peroxidase-like activity of CuO nanoparticles, which effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals. The effects of the reactant concentrations and some organic compounds were also investigated. The proposed method could be used as a sensitive detection tool for hydrogen peroxide and glucose.
作为过氧化物酶的模拟物，氧化铜纳米粒子被发现可将鲁米诺-H2O2 体系的化学发光（CL）增强高达 400 倍。通过进行 CL 光谱和自由基清除剂实验，研究了可能的 CL 增强机制。研究表明，增强的 CL 可归因于 CuO 纳米粒子的过氧化物酶样活性，其可有效催化过氧化氢分解为羟基自由基。还研究了反应物浓度和一些有机化合物的影响。该方法可用于检测过氧化氢和葡萄糖。
