Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan.
J Agric Food Chem. 2010 Mar 24;58(6):3847-54. doi: 10.1021/jf904563c.
Ginger, the rhizome of Zingiber officinale , is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.
生姜是姜属植物的根茎，作为一种传统药物，具有祛风、止呕、抗炎和抗癌等功效。本研究检测了生姜中一种辛辣酚类化合物[8]-姜烯酚对人白血病 HL-60 细胞的生长抑制作用。结果表明，[8]-姜烯酚能以时间和浓度依赖的方式诱导细胞凋亡。[8]-姜烯酚处理会导致线粒体跨膜电位迅速丧失，活性氧（ROS）生成增加，线粒体细胞色素 c 释放到细胞质中，随后诱导前胱冬酶-9 和前胱冬酶-3 加工。综上所述，这些结果首次表明，ROS 的产生和谷胱甘肽的耗竭导致了[8]-姜烯酚诱导 HL-60 细胞凋亡。
