[8]-姜烯酚通过活性氧产生、谷胱甘肽耗竭和半胱氨酸天冬氨酸蛋白酶激活诱导人白血病细胞凋亡。
Induction of apoptosis by [8]-shogaol via reactive oxygen species generation, glutathione depletion, and caspase activation in human leukemia cells.
机构信息
Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan.
出版信息
J Agric Food Chem. 2010 Mar 24;58(6):3847-54. doi: 10.1021/jf904563c.
Abstract
Ginger, the rhizome of Zingiber officinale , is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.
摘要
生姜是姜属植物的根茎，作为一种传统药物，具有祛风、止呕、抗炎和抗癌等功效。本研究检测了生姜中一种辛辣酚类化合物[8]-姜烯酚对人白血病 HL-60 细胞的生长抑制作用。结果表明，[8]-姜烯酚能以时间和浓度依赖的方式诱导细胞凋亡。[8]-姜烯酚处理会导致线粒体跨膜电位迅速丧失，活性氧（ROS）生成增加，线粒体细胞色素 c 释放到细胞质中，随后诱导前胱冬酶-9 和前胱冬酶-3 加工。综上所述，这些结果首次表明，ROS 的产生和谷胱甘肽的耗竭导致了[8]-姜烯酚诱导 HL-60 细胞凋亡。
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