Kacem Mohamed, Simon Gaëlle, Leschiera Raphael, Misery Laurent, ElFeki Abdelfattah, Lebonvallet Nicolas
Laboratory of Animal Ecophysiology, Life Sciences Department, Sfax Faculty of Science, University of Sfax, BP1171, 3000, Sfax, Tunisia,
In Vitro Cell Dev Biol Anim. 2015 Feb;51(2):128-41. doi: 10.1007/s11626-014-9813-7. Epub 2014 Oct 2.
Ruta chalepensis L. is used in the traditional herbal treatment of various diseases. The aim of this work is to investigate the effect of different extracts of R. chalepensis L. on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expressions and their antioxidant capacity on murine RAW 264.7 macrophage challenged with lipopolysaccharide (LPS). In fact, this study shows that the ethanol and ethyl acetate extracts of R. chalepensis L. considerably decreased the nitric oxide (NO) production in murine RAW 264.7 macrophages stimulated with lipopolysaccharide. Thus, the treatment with both extracts significantly suppressed the levels of iNOS and COX-2 gene expressions through the inhibition of the nuclear factor-κB (NF-κB) activation. The preincubation of RAW 264.7 cells with various concentrations of ethanol and ethyl acetate extracts decreased the production of thiobarbituric acid-reactive substances (TBARS) in a dose-dependent manner. It also increased the activities of antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in LPS-stimulated macrophages, compared to those in the cells treated only with LPS. Besides, the (1)H NMR spectra of both extracts have demonstrated the presence of aromatic signals, thus confirming the existence of phenolic compounds such as flavonoids and polyphenols. So, the ethanol and ethyl acetate extracts of R. chalepensis L. have been shown to possess enough antioxidant and anti-inflammatory activities to prevent LPS-induced oxidative stress and inflammation in RAW 264.7 macrophages.
白藓（Ruta chalepensis L.）被用于多种疾病的传统草药治疗。本研究的目的是调查白藓不同提取物对脂多糖（LPS）刺激的小鼠RAW 264.7巨噬细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）基因表达的影响及其抗氧化能力。事实上，本研究表明，白藓的乙醇提取物和乙酸乙酯提取物能显著降低脂多糖刺激的小鼠RAW 264.7巨噬细胞中一氧化氮（NO）的产生。因此，两种提取物的处理均通过抑制核因子-κB（NF-κB）的激活，显著抑制了iNOS和COX-2基因的表达水平。用不同浓度的乙醇提取物和乙酸乙酯提取物对RAW 264.7细胞进行预孵育，可剂量依赖性地降低硫代巴比妥酸反应性物质（TBARS）的产生。与仅用LPS处理的细胞相比，它还增加了LPS刺激的巨噬细胞中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等抗氧化酶的活性。此外，两种提取物的核磁共振氢谱（1H NMR）均显示出芳香信号，从而证实了黄酮类和多酚类等酚类化合物的存在。因此，白藓的乙醇提取物和乙酸乙酯提取物已被证明具有足够的抗氧化和抗炎活性，可预防LPS诱导的RAW 264.7巨噬细胞氧化应激和炎症。
