Livestock Experiment Station, Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Daisen, Japan.
Anim Sci J. 2009 Apr;80(2):220-3. doi: 10.1111/j.1740-0929.2008.00624.x.
Recently, we reported a method for discriminating a Japanese brand of chicken, the Hinai-jidori. As an application of this method for discriminating Hinai-jidori eggs, we here report an efficient method for extracting maternal DNA from eggshells. Eggshell powder was completely decalcified with EDTA solution, and then DNA was isolated by conventional phenol-chloroform extraction and ethanol precipitation. The efficiency of DNA recovery from eggshells was 50-fold higher than that of a previously reported method. The recovered DNA could be used for PCR, and 10 markers for identifying the Hinai-jidori chicken were detected. The genotypes of the Hinai-jidori exactly matched those of the Hinai-dori breed. Using this method, Hinai-jidori and Hinai-dori eggs could be distinguished from the eggs of Rhode Island Reds. This is the first report of a technique that can be used to discriminate the eggs of Hinai-jidori from those of other chickens, and it can also be utilized to validate the labeling of Hinai-jidori eggs in the market.
最近，我们报道了一种鉴别日本品牌鸡——日野鸡的方法。作为该方法在鉴别日野鸡蛋中的应用，我们在此报告一种从蛋壳中提取母系 DNA 的有效方法。蛋壳粉末用 EDTA 溶液完全脱钙，然后通过常规的酚-氯仿提取和乙醇沉淀分离 DNA。从蛋壳中回收 DNA 的效率比以前报道的方法高 50 倍。回收的 DNA 可用于 PCR，检测到 10 个用于鉴定日野鸡的标记。日野鸡的基因型与日野鸡品种完全匹配。使用这种方法，可以将日野鸡蛋与罗得岛红鸡的鸡蛋区分开来。这是首次报道一种可用于鉴别日野鸡蛋与其他鸡种鸡蛋的技术，也可用于验证市场上日野鸡蛋的标签。
