Fiber-Nanotech Young Researcher Empowerment Center, Shinshu University, Minamiminowa, Kamiina, Japan.
Anim Sci J. 2009 Oct;80(5):597-604. doi: 10.1111/j.1740-0929.2009.00680.x.
Immunostimulatory sequences of oligodeoxynucleotides (ODNs), such as CpG ODNs, are potent stimulators of innate immunity. Here, we identified a strong immunostimulatory CpG ODN, which we named MsST, from the lac Z gene of Streptococcus (S.) thermophilus ATCC19258, and we evaluated its immune functions. In in vitro studies, MsST had a similar ability as the murine prototype CpG ODN 1555 to induce inflammatory cytokine production and cell proliferation. In mouse splenocytes, MsST increased the number of CD80+CD11c+and CD86+CD11c+ dendritic cells and CD4+CD25+ regulatory T cells. We also analyzed the effects of MsST on the expression of regulatory cytokines by real-time quantitative PCR. MsST was more potent at inducing interleukin-10 expression than the ODN control 1612, indicating that MsST can augment the regulatory T cell response via Toll-like receptor 9, which plays an important role in suppressing T helper type 2 responses. These results suggest that S. thermophilus, whose genes include a strong Immunostimulatory sequence-ODN, is a good candidate for a starter culture to develop new physiologically functional foods and feeds.
寡脱氧核苷酸（ODN）的免疫刺激序列，如 CpG ODN，是先天免疫的有效刺激物。在这里，我们从嗜热链球菌（S.）ATCC19258 的 lac Z 基因中鉴定出一种强免疫刺激 CpG ODN，我们将其命名为 MsST，并评估了其免疫功能。在体外研究中，MsST 具有与鼠原型 CpG ODN 1555 相似的诱导炎症细胞因子产生和细胞增殖的能力。在小鼠脾细胞中，MsST 增加了 CD80+CD11c+和 CD86+CD11c+树突状细胞和 CD4+CD25+调节性 T 细胞的数量。我们还通过实时定量 PCR 分析了 MsST 对调节性细胞因子表达的影响。与 ODN 对照 1612 相比，MsST 更能诱导白细胞介素-10 的表达，这表明 MsST 可以通过 Toll 样受体 9 增强调节性 T 细胞反应，Toll 样受体 9 在抑制辅助性 T 细胞 2 型反应中起重要作用。这些结果表明，包含强免疫刺激序列-ODN 的嗜热链球菌是开发新型生理功能食品和饲料的起始培养物的良好候选物。
