Graduate School of Agriculture, Shinshu University, Minamiminowa 399-4598, Japan.
FEBS Open Bio. 2012 Nov 12;3:41-5. doi: 10.1016/j.fob.2012.11.002. Print 2013.
We designed class I/II hybrid inhibitory oligodeoxynucleotides (iODNs), called iSG, and found that the sequence 5'-TTAGGG-3', which has a six-base loop head structure, and a 3'-oligo (dG)3-5 tail sequence are important for potent immunosuppressive activity. Interestingly, splenocytes isolated from ovalbumin (OVA)-immunized mice and treated with iSG3 showed suppression of not only interleukin (IL)-6, IL-12p35, IL-12p40, and interferon (IFN) γ mRNA expression, but also IL-4 and IL-13 mRNA expression. Thus, both Th2 and Th1 immune responses can be strongly suppressed by iODNs in splenocytes from allergen-immunized mice, suggesting usefulness in the treatment of diseases induced by over-active immune activation.
我们设计了一种称为 iSG 的 I/II 型混合抑制性寡脱氧核苷酸 (iODN)，并发现具有六碱基环头结构的 5'-TTAGGG-3'序列和 3'-寡 (dG)3-5'尾序列对于强效免疫抑制活性很重要。有趣的是，用 iSG3 处理卵清蛋白 (OVA) 免疫小鼠分离的脾细胞不仅抑制白细胞介素 (IL)-6、IL-12p35、IL-12p40 和干扰素 (IFN) γ mRNA 的表达，还抑制 IL-4 和 IL-13 mRNA 的表达。因此，iODN 可强烈抑制过敏原免疫小鼠脾细胞中的 Th2 和 Th1 免疫反应，提示在治疗过度活跃的免疫激活引起的疾病方面具有应用价值。
