Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchui, Tokyo, Japan.
Anim Sci J. 2009 Jun;80(3):322-7. doi: 10.1111/j.1740-0929.2009.00639.x.
Peroxisome proliferatior-activated receptor gamma (PPARgamma) is a transcription factor that regulates adipocyte differentiation, and the activation of PPARgamma increases fat deposition in growing chickens. The aim of the present study was to investigate whether the levels of PPARgamma gene expression were related to fat pad weight in abdominal adipose tissue in growing chickens with different genotype and sex. Body weight and abdominal adipose tissue weight in broiler chickens (Ross strain) were higher than the other genotypes (Road Island Red, White Leghorn, and Japanese native poultry (Tsushima)) at 3 and 5 weeks of age. PPARgamma mRNA expression in abdominal adipose tissue tended to increase with age, as evidenced by higher expression levels at 5 weeks than at 1 week of age in all sex and genotype of chickens. In broiler chickens, the PPARgamma expressions were significantly higher than the other genotypes. PPARgamma mRNA expression levels in abdominal adipose tissue of female chickens rapidly increased at 3 weeks, and were unchanged until 5 weeks, while those in male chickens gradually increased until 5 weeks. In addition, abdominal adipose tissue weight was correlated with PPARgamma expression levels. These results demonstrated that PPARgamma gene expression is a useful marker of fat deposition in chickens, suggesting that PPARgamma is a key factor of fat accumulation in chicken abdominal fat pad.
过氧化物酶体增殖物激活受体 γ（PPARγ）是一种转录因子，可调节脂肪细胞分化，激活 PPARγ会增加生长鸡的脂肪沉积。本研究旨在探讨不同基因型和性别的生长鸡中，PPARγ基因表达水平与腹部脂肪垫中脂肪垫重量的关系。3 周和 5 周龄时，肉鸡（罗斯品种）的体重和腹部脂肪组织重量均高于其他基因型（罗得岛红、白来航和日本土鸡（对马））。腹部脂肪组织中 PPARγ mRNA 的表达随年龄的增长而趋于增加，所有性别和基因型的鸡在 5 周龄时的表达水平均高于 1 周龄。在肉鸡中，PPARγ 的表达明显高于其他基因型。3 周龄时，母鸡腹部脂肪组织中的 PPARγ mRNA 表达迅速增加，直到 5 周龄时保持不变，而公鸡中的表达则逐渐增加，直到 5 周龄。此外，腹部脂肪组织重量与 PPARγ 表达水平相关。这些结果表明，PPARγ 基因表达是鸡体脂肪沉积的有用标志物，提示 PPARγ 是鸡腹脂垫脂肪积累的关键因素。
