Gomes D E, Hirata K Y, Saheki K, Rosa A C G, Luvizotto M C R, Cardoso T C
Laboratory of Animal Virology, DAPSA, Brazil.
J Comp Pathol. 2010 Jul;143(1):8-13. doi: 10.1016/j.jcpa.2009.12.012. Epub 2010 Feb 16.
Twenty 1-day-old specific pathogen free chicks and 20 1-day-old commercially derived turkey poults were inoculated with a Brazilian strain of turkey coronavirus (TCoV) to study the pathogenicity and virus distribution up to 14 days post-inoculation by histopathology, immunohistochemistry, reverse transcriptase polymerase chain reaction and sequencing. At 2-14 dpi, TCoV antigens were detected in the paranasal sinus and lachrymal accessory gland (Harderian gland) of infected chicks and in the ileum, ileocaecal junction and caecum of infected poults. Lymphocytic inflammation was present in these tissues. TCoV was re-isolated from pooled tissue suspensions of nasal concha, Harderian gland and paranasal sinus from chicks, as well as from the ileum, ileocaecal junction and caecum of poults, after three consecutive passages in 28-day-old embryonated turkey eggs. Viral RNA corresponding to the spike gene region (1178-2073 genome position) was amplified from the upper respiratory tract of chickens and from the intestinal tract of poults and phylogenetic analysis confirmed the identity as TCoV. This is the first description of TCoV antigens and mRNA in upper respiratory tissues in experimentally infected chickens.
选用20只1日龄无特定病原体雏鸡和20只1日龄商业来源火鸡雏，接种巴西株火鸡冠状病毒（TCoV），通过组织病理学、免疫组织化学、逆转录聚合酶链反应和测序研究接种后14天内的致病性和病毒分布。在接种后2至14天，在感染雏鸡的鼻旁窦和泪腺（哈德氏腺）以及感染火鸡雏的回肠、回盲交界处和盲肠中检测到TCoV抗原。这些组织中存在淋巴细胞性炎症。在28日龄鸡胚中连续传代三次后，从雏鸡的鼻甲、哈德氏腺和鼻旁窦以及火鸡雏的回肠、回盲交界处和盲肠的混合组织悬液中重新分离出TCoV。从鸡的上呼吸道和火鸡雏的肠道中扩增出与刺突基因区域（基因组位置1178 - 2073）对应的病毒RNA，系统发育分析证实其为TCoV。这是首次描述实验感染鸡上呼吸道组织中的TCoV抗原和mRNA。
