Marine Science Program, Department of Biological Sciences, University of South Carolina, 715 Sumter St., Columbia, SC 29208, USA.
Water Res. 2010 Apr;44(8):2461-72. doi: 10.1016/j.watres.2010.01.012. Epub 2010 Jan 29.
The utility of a multiple-fixed-wavelength spectral fluorometer, the Algae Online Analyser (AOA), as a means of quantifying phytoplankton biomass and community composition was tested using natural communities from two southeastern United States estuaries, North Inlet, South Carolina, and the Neuse River Estuary, North Carolina. Estimates of biomass (as chlorophyll a) were correlated with HPLC values and variations (usually over-estimates) were consistent with effects of light intensity and nutrient availability on fluorescence quenching. AOA estimates of taxonomic structure were consistent with those from HPLC-derived marker pigments by ChemTax, with both methods indicating domination by chromophytes and green algae in North Inlet and chromophytes and cyanobacteria in the Neuse. We recommend frequent calibration by discrete sample collection, and calibration with species representative of the region of interest. Overall, the AOA appears to be a useful tool for monitoring of phytoplankton community composition, especially as an early warning system for the detection of harmful algal blooms.
利用多固定波长光谱荧光计（AOA）定量分析浮游植物生物量和群落组成的实用性，在两个美国东南部的河口，南卡罗来纳州的北进港和北卡罗来纳州的内斯河河口的自然群落中进行了测试。生物量（以叶绿素 a 计）的估计值与 HPLC 值相关，变化（通常是高估）与光强和营养可用性对荧光猝灭的影响一致。AOA 对分类结构的估计与 HPLC 衍生的标记色素 ChemTax 的结果一致，这两种方法都表明在北进港以色素体和绿藻为主，在内斯河以色素体和蓝藻为主。我们建议通过离散样本采集进行频繁的校准，并使用代表目标区域的物种进行校准。总的来说，AOA 似乎是监测浮游植物群落组成的有用工具，尤其是作为有害藻华检测的早期预警系统。
