通过毛细管气相色谱法直接测定几丁质及含几丁质生物制品强酸性水解产物中的乙酸。
Direct determination of acetic acid in strongly acidic hydrolysates of chitin and chitin-containing biological products by capillary gas chromatography.
作者信息
Stoev G, Velichkov A
机构信息
Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia.
出版信息
J Chromatogr. 1991 Feb 1;538(2):431-4. doi: 10.1016/s0021-9673(01)88864-6.
PMID:2016386
Abstract
Laboratory-made capillary columns with high effectiveness and inertness were used for the determination of N-acetyl groups in chitin-containing biological products by liberation of acetic acid in 4 M HCl hydrolysates. The quantitative gas chromatographic analysis is fast, requiring only 2 min, with good precision and accuracy. After a long working period of more than 1000 analyses the capillary column retained its high effectiveness and inertness.
摘要
采用高效、惰性的实验室自制毛细管柱，通过在4M盐酸水解产物中释放乙酸来测定含几丁质生物制品中的N-乙酰基。定量气相色谱分析速度快，仅需2分钟，精密度和准确度良好。经过1000多次分析的长时间使用后，毛细管柱仍保持其高效性和惰性。
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