Henry R J, Blakeney A B, Harris P J, Stone B A
J Chromatogr. 1983 Feb 18;256(3):419-27. doi: 10.1016/s0021-9673(01)88259-5.
A new method for the preparation and separation of alditol acetates from neutral sugars has been applied to aminosugars. Reduced aminosugars were rapidly acetylated using 1-methylimidazole as the catalyst without removal of borate formed during reduction. The alditol acetates were separated by glass capillary gas chromatography on Silar 10C. The alditol acetates of aminosugars had retention times much longer than those of neutral sugars. However, the alditol acetates of the deamination products of aminosugars had shorter retention times and were resolved from those of neutral sugars. This method was used for the simultaneous detection of neutral and aminosugars in acid hydrolysates of chitin and the glycoproteins, ovalbumin and peroxidase.
一种从中性糖制备和分离糖醇乙酸酯的新方法已应用于氨基糖。使用1-甲基咪唑作为催化剂，还原后的氨基糖可快速乙酰化，无需去除还原过程中形成的硼酸盐。糖醇乙酸酯通过在Silar 10C上的玻璃毛细管气相色谱法进行分离。氨基糖的糖醇乙酸酯的保留时间比中性糖长得多。然而，氨基糖脱氨产物的糖醇乙酸酯的保留时间较短，并与中性糖的糖醇乙酸酯分离。该方法用于同时检测几丁质和糖蛋白、卵清蛋白及过氧化物酶的酸水解产物中的中性糖和氨基糖。
