心脏手术期间采集的中性粒细胞上Apo/Fas（CD95）复合物的上调：使用或不使用体外循环进行手术的患者中的不同发现。

Up-regulation of the Apo/Fas (CD95) complex on neutrophils harvested during cardiac surgery: distinct findings in patients operated on with or without the use of cardiopulmonary bypass.

作者信息

Kunes Pavel, Mandak Jiri, Harrer Jan, Kolackova Martina, Andrys Ctirad, Holicka Monika, Krejsek Jan

机构信息

Department of Cardiac Surgery, Charles University in Prague, University Hospital and Medical Faculty in Hradec Kralove, Hradec Kralove, Czech Republic.

出版信息

Perfusion. 2010 Jan;25(1):41-6. doi: 10.1177/0267659110363318. Epub 2010 Feb 17.

DOI:10.1177/0267659110363318

PMID:20164160

Abstract

AIMS

In a group of patients undergoing cardiac surgery performed both with ("on-pump") and without the use ("offpump") of cardiopulmonary bypass (CPB), we studied the changes of neutrophil membrane apoptosis-inducing complex Apo/Fas.

METHODS

Expression of Apo/Fas (CD95) on leukocytes was evaluated by flow cytometry.

RESULTS

In "on-pump" patients, we found an increase in the expression of CD95 median intensity fluorescence (MFI) on granulocytes from a baseline level median=56, (Q( 1)=45.5, Q(3)=64) to a median=88, (Q(1)=62, Q( 3)=109.5; p<0.01) at the 3(rd) postoperative day and median=74, (Q(1)=63, Q(3)=84.5; p<0.01) at the 7(th) postoperative day. In "off-pump" patients, granulocyte CD95 MFI was median=55, (Q(1)=51, Q(3)=84) before surgery. The significant increase was found on the 3(rd) postoperative day only; median=90, (Q( 1)=66; Q(3)=98; p<0.05). A similar pattern in the CD95 expression was also found if percentage changes of granulocyte CD95 MFI were followed. Moreover, the significantly increased Apo/Fas expression expressed as a percentage change of CD95 MFI was found in "on-pump" patients compared to "off-pump" patients, both at the 3(rd) postoperative day (p<0.05) and at the 7(th) postoperative day (p<0.01).

CONCLUSIONS

This is the first direct evidence of increasing densities of the Apo/Fas complex on neutrophils in cardiac surgical patients.

摘要

目的

在一组接受心脏手术的患者中，我们研究了在使用体外循环（“on-pump”）和不使用体外循环（“off-pump”）两种情况下，中性粒细胞膜凋亡诱导复合物Apo/Fas的变化。

方法

通过流式细胞术评估白细胞上Apo/Fas（CD95）的表达。

结果

在“on-pump”患者中，我们发现粒细胞上CD95中位强度荧光（MFI）表达从基线水平中位数=56（Q(1)=45.5，Q(3)=64）增加到术后第3天的中位数=88（Q(1)=62，Q(3)=109.5；p<0.01）以及术后第7天的中位数=74（Q(1)=63，Q(3)=84.5；p<0.01）。在“off-pump”患者中，术前粒细胞CD95 MFI中位数=55（Q(1)=51，Q(3)=84）。仅在术后第3天发现显著增加；中位数=90（Q(1)=66；Q(3)=98；p<0.05）。如果追踪粒细胞CD95 MFI的百分比变化，在CD95表达上也发现了类似模式。此外，与“off-pump”患者相比，“on-pump”患者在术后第3天（p<0.05）和术后第7天（p<0.01），以CD95 MFI百分比变化表示的Apo/Fas表达均显著增加。