Department of Ophthalmology, University of Duisburg-Essen, Hufelandstrasse 55, Essen, Germany.
Cornea. 2010 Apr;29(4):462-4. doi: 10.1097/ICO.0b013e3181b442ea.
To report a case of partial limbal stem cell deficiency (LSCD) caused by epidermolysis bullosa dystrophica mutilans Hallopeau-Siemens treated by transplantation of autologous ex vivo expanded limbal epithelium.
Review of the clinical findings of an 11.5-year-old boy with unilateral LSCD and epidermolysis bullosa dystrophica who underwent ocular surface reconstruction in the right eye with autologous on intact human amniotic membrane cultivated limbal epithelial cells.
Twenty-eight months after reconstruction, the corneal surface is clear, smooth, and stable showing no signs of LSCD recurrence. Three subconjunctival bevacizumab (Avastin) injections reduced the recurrent growth of symblepharon and corneal vascularization. The visual acuity has increased from hand motion to 20/50.
Autologous transplantation of cultivated human limbal epithelial cells on intact human amniotic membrane can be a safe and effective method for corneal surface reconstruction in LSCD caused by recessive epidermolysis bullosa dystrophica.
报告 1 例由 Hallopeau-Siemens 型营养不良性大疱性表皮松解症引起的部分角膜缘干细胞缺乏症（LSCD），采用自体体外扩增的角膜缘上皮移植进行治疗。
回顾性分析 11.5 岁单侧 LSCD 合并营养不良性大疱性表皮松解症患儿的临床资料，该患儿右眼接受了自体同源完整人羊膜培养的角膜缘上皮细胞眼表重建。
重建术后 28 个月，角膜表面清晰、光滑且稳定，无 LSCD 复发迹象。3 次球结膜下注射贝伐单抗（Avastin）可减少复发性睑球粘连和角膜血管化。视力从手动提高至 20/50。
自体同源完整人羊膜培养的角膜缘上皮细胞移植可为隐性营养不良性大疱性表皮松解症引起的 LSCD 患者的角膜表面重建提供一种安全有效的方法。
