Department of Microbial Ecology, Netherlands Institute of Ecology , Heteren, The Netherlands.
ISME J. 2010 May;4(5):711-5. doi: 10.1038/ismej.2010.11. Epub 2010 Feb 18.
Although secondary succession has been studied extensively, we have little knowledge of the succession of soil-borne microbial communities. In this study, we therefore examined the structures of the microbial communities across two separate chronosequences of chalk grasslands in Limburg, the Netherlands, which are at different stages of secondary succession after being abandoned for between 17 and >66 years. Arable fields were also included in the investigation as non-abandoned references. Changes in the soil-borne microbial communities, as determined by phylogenetic microarray and quantitative PCR methodologies, were correlated with the prevailing environmental conditions related to vegetation and soil biochemistry. We observed clear patterns of microbial secondary succession related to soil age, pH and phosphate status, as exemplified by the overrepresentation of Verrucomicrobia, Acidobacteria, Gemmatimonadetes, and alpha-, delta- and epsilon-Proteobacteria at late successional stages. Moreover, effects of secondary succession versus changes in soil pH could be resolved, with pH significantly altering the trajectory of microbial succession.
尽管已经对次级演替进行了广泛的研究，但我们对土壤传播微生物群落的演替知之甚少。因此，在本研究中，我们检查了荷兰林堡两个不同 chalk grasslands 的微生物群落结构，这些 chalk grasslands 因废弃时间在 17 至>66 年之间而处于不同的次级演替阶段。耕地也被包括在调查中作为非废弃的参考。通过系统发育微阵列和定量 PCR 方法确定的土壤传播微生物群落的变化与与植被和土壤生物化学有关的流行环境条件相关。我们观察到与土壤年龄、pH 值和磷酸盐状态相关的微生物次级演替的明显模式，例如在晚期演替阶段，疣微菌门、酸杆菌门、芽单胞菌门以及α-、δ-和ε-变形菌门的过度表达。此外，可以解决次级演替与土壤 pH 值变化的影响，pH 值显著改变了微生物演替的轨迹。
