Zhu Y, Hayakawa T, Toriyama S, Takahashi M
Plantech Research Institute, Yokohama-city, Japan.
J Gen Virol. 1991 Apr;72 ( Pt 4):763-7. doi: 10.1099/0022-1317-72-4-763.
The RNA 3 of rice stripe virus (RSV) isolate T was cloned and its nucleotide sequence was determined. The complete primary structure was found to consist of 2504 nucleotides. One putative open reading frame (ORF), between nucleotides 2412 and 1444 in the 5'-proximal region of the virus complementary-sense RNA, encoded a 322 amino acid protein with an Mr of 35,134 that was identified as the coat protein. The other ORF, between nucleotides 66 and 701 in the 5'-proximal region of the virus-sense RNA, encoded a 211 amino acid protein with an Mr of 23,874. An intergenic non-coding region (742 nucleotides) between the two ORFs contains oligo(A)- and oligo(U)-rich regions that can be arranged into a 126 base pair stem configuration. These results suggest that RSV RNA 3 has an ambisense coding strategy.
克隆了水稻条纹病毒（RSV）分离株T的RNA 3，并测定了其核苷酸序列。发现其完整的一级结构由2504个核苷酸组成。在病毒互补义RNA 5'近端区域的核苷酸2412和1444之间有一个推定的开放阅读框（ORF），编码一个含有322个氨基酸、分子量为35134的蛋白质，该蛋白质被鉴定为外壳蛋白。另一个ORF在病毒义RNA 5'近端区域的核苷酸66和701之间，编码一个含有211个氨基酸、分子量为23874的蛋白质。两个ORF之间的基因间隔非编码区（742个核苷酸）包含富含寡聚腺苷酸和寡聚尿苷酸的区域，这些区域可排列成126个碱基对的茎环结构。这些结果表明RSV RNA 3具有双义编码策略。
