Kakutani T, Hayano Y, Hayashi T, Minobe Y
Department of Molecular Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
J Gen Virol. 1990 Jul;71 ( Pt 7):1427-32. doi: 10.1099/0022-1317-71-7-1427.
The complete nucleotide sequence of segment 4 of the rice stripe virus (RSV) genome was determined from overlapping cDNA clones and by direct RNA sequencing. The segment has two long open reading frames (ORFs). One of the ORFs (534 bases) is in the 5' region of the viral sequence, and the other (858 bases) is in the viral complementary sequence of the viral 3' region. Such ambisense genome organizations have been seen previously in RNAs of phleboviruses, uukuviruses and arenaviruses. The ORF in the viral sequence encodes the major non-structural protein, but the product of the ORF in the viral complementary sequence (Mr 32407) has not yet been identified. The first 18 bases of each of the 5' and 3' ends of segment 4 are complementary in sequence. Among the 3'-terminal 16 bases, 14 were identical between RSV genome segments 3 and 4. These terminal sequences are very similar to those of RNAs of phleboviruses and uukuviruses. These and other characteristics indicate a possible evolutionary relationship between RSV and phleboviruses and/or uukuviruses.
通过重叠cDNA克隆和直接RNA测序确定了水稻条纹病毒（RSV）基因组第4节段的完整核苷酸序列。该节段有两个长开放阅读框（ORF）。其中一个ORF（534个碱基）位于病毒序列的5'区域，另一个（858个碱基）位于病毒3'区域的病毒互补序列中。这种双义基因组结构先前已在白蛉病毒、乌库病毒和沙粒病毒的RNA中观察到。病毒序列中的ORF编码主要的非结构蛋白，但病毒互补序列中ORF的产物（Mr 32407）尚未确定。第4节段5'和3'末端的前18个碱基在序列上互补。在3'末端的16个碱基中，RSV基因组第3和第4节段有14个相同。这些末端序列与白蛉病毒和乌库病毒的RNA末端序列非常相似。这些以及其他特征表明RSV与白蛉病毒和/或乌库病毒之间可能存在进化关系。
