Soltan Hamdy R, Abd El-Megeed Fawkia A, Mustafa Fathia I, Ismail Hanafy M
Alexandria University, Faculty of Agriculture, Pesticide Chemistry Department, PO Box 21545, Alexandria, Egypt.
J AOAC Int. 2009 Nov-Dec;92(6):1815-20.
A sensitive and selective indirect competitive ELISA was developed for the detection of deltamethrin bound residues on cotton texture. Cross-reactivity studies with the main deltamethrin photodegradation products showed high specificity of deltamethrin polyclonal antibody to the parent compound. No cross-reactivity was measured with deltamethrin photodegradtion products derived from the alcohol moiety (3-phenoxybenzaldhyde, phenoxybenzyl alcohol, cyanohydrin, and 3-phenoxybenzoic acid), and lesser amounts were observed with the acid moiety (deltamethric acid). The dot-blot immunoassay was performed on cotton fabric discs spiked with deltamethrin and irradiated to assess the suitability of this system to detect bound residue. The dot-blot immunoassay results revealed that the bound form of deltamethrin has binding affinity with deltamethrin antibody similar to the parent compound. In addition, the test system was used to detect bound and free residues of deltamethrin on cotton samples exposed to three cycles of simulated sunlight and water wash. The results obtained suggest that the competitive ELISA format can be used as a tool for monitoring free and bound residues of deltamethrin impregnated on cotton targets.
开发了一种灵敏且具选择性的间接竞争酶联免疫吸附测定法（ELISA），用于检测棉织物上溴氰菊酯的结合残留。与主要溴氰菊酯光降解产物的交叉反应研究表明，溴氰菊酯多克隆抗体对母体化合物具有高度特异性。未检测到与醇部分（3 - 苯氧基苯甲醛、苯氧基苄醇、氰醇和3 - 苯氧基苯甲酸）衍生的溴氰菊酯光降解产物有交叉反应，而与酸部分（溴氰菊酸）的交叉反应较少。对添加了溴氰菊酯并经辐照的棉织物圆盘进行斑点印迹免疫测定，以评估该系统检测结合残留的适用性。斑点印迹免疫测定结果表明，溴氰菊酯的结合形式与母体化合物类似，对溴氰菊酯抗体具有结合亲和力。此外，该测试系统用于检测暴露于三个模拟阳光和水洗循环的棉花样品上溴氰菊酯的结合和游离残留。结果表明，竞争ELISA形式可作为监测浸渍在棉花靶标上溴氰菊酯的游离和结合残留的工具。
