Department of Molecular Science and Technology, Ajou University, 5 Wonchon, Yeoungtong, Suwon 443-749, Republic of Korea.
Biomacromolecules. 2010 Mar 8;11(3):617-25. doi: 10.1021/bm100047y.
Fast in situ forming supramolecular hydrogels consisted of the tyramine-conjugated supramolecular structures and chitosan derivative were prepared via an enzymatic reaction with horseradish peroxidase (HRP) and hydrogen peroxide (H(2)O(2)). The gel formation was varied within a time period of 5 s to 10 min by controlling the concentrations of HRP, H(2)O(2), and polymers. Tyramine conjugation at different sites of the supramolecular structure resulted in significant changes in physical properties and the degradation time of the hydrogels that were confirmed by water uptake, compressive strength and degradation tests. In addition, the hydrogels showed a good cytocompatibility in vitro. These hydrogels could be promising injectable biomaterials with adjustable degradation times to control both the cellular behaviors as a regenerative cell matrix and the drug release behavior as a drug delivery vehicle.
通过辣根过氧化物酶（HRP）和过氧化氢（H₂O₂）的酶反应，制备了由结合了酪胺的超分子结构和壳聚糖衍生物组成的快速原位形成的超分子水凝胶。通过控制 HRP、H₂O₂ 和聚合物的浓度，凝胶的形成时间可在 5 秒至 10 分钟内变化。超分子结构的不同部位的酪胺结合导致水凝胶的物理性质和降解时间发生显著变化，这通过吸水率、压缩强度和降解试验得到了证实。此外，水凝胶在体外表现出良好的细胞相容性。这些水凝胶可能是有前途的可注射生物材料，具有可调节的降解时间，以控制细胞行为作为再生细胞基质和药物释放行为作为药物输送载体。
