Department of Molecular Science and Technology, Ajou University, 5 Wonchon, Yeoungtong, Suwon 443-749, Republic of Korea.
Biomacromolecules. 2011 Aug 8;12(8):2872-80. doi: 10.1021/bm200326g. Epub 2011 Jun 27.
An in situ gel-forming system composed of rutin- and tyramine-conjugated chitosan derivatives, horseradish peroxidase (HRP), and hydrogen peroxide (H(2)O(2)) was prepared and applied to dermal wound repair. Rutin was employed to enhance production and accumulation of extracellular matrix in the healing process. In vitro study demonstrates that released rutin significantly enhanced cell proliferation as compared with media without rutin. In vivo wound healing study was performed by injecting hydrogels on rat dorsal wounds with a diameter of 8 mm for 14 days. Histological results demonstrated that rutin-conjugated hydrogel exhibited enhancement of wound healing as compared with treatments with PBS, hydrogel without rutin, and a commercialized wound dressing (Duoderm). More specifically, rutin-conjugated hydrogels induced better defined formation of neo-epithelium and thicker granulation, which is closer to the original epithelial tissue. As a result, this study suggests that the in situ gel-forming system can be a promising injectable gel-type wound dressing.
一种由芦丁和酪胺修饰壳聚糖衍生物、辣根过氧化物酶（HRP）和过氧化氢（H₂O₂）组成的原位凝胶形成系统被制备并应用于皮肤伤口修复。芦丁被用于增强细胞外基质在愈合过程中的产生和积累。体外研究表明，与不含芦丁的培养基相比，释放的芦丁可显著促进细胞增殖。通过在大鼠背部直径为 8 毫米的伤口上注射水凝胶进行体内伤口愈合研究，共进行 14 天。组织学结果表明，与 PBS、不含芦丁的水凝胶和商业化的伤口敷料（Duoderm）相比，芦丁修饰的水凝胶可显著增强伤口愈合。更具体地说，芦丁修饰的水凝胶诱导了更好的新生上皮形成和更厚的肉芽组织，这更接近原始的上皮组织。因此，本研究表明，该原位凝胶形成系统可以作为一种有前途的可注射凝胶型伤口敷料。
