评估双核铜（II）配合物的糖苷水解活性。
Evaluating binuclear copper(II) complexes for glycoside hydrolysis.
机构信息
Department of Chemistry and Biochemistry, 179 Chemistry Building, Auburn University, Auburn, Alabama 36849, USA.
出版信息
Inorg Chem. 2010 Mar 15;49(6):2639-48. doi: 10.1021/ic9014064.
Three binuclear copper(II) complexes were characterized as solids by X-ray diffraction and in solution by UV/vis spectrophotometric titration, and subsequently evaluated for their glycosidase-like activity. The structure analysis revealed comparable intermetallic Cu...Cu distances (approximately 3.5 A) for the complexes 2 and 3. Despite this similarity, the composition of the complexes differs significantly in aqueous solution as revealed by spectrophotometric titrations. The hydrolysis of selected nitrophenylglycopyranosides is up to 11,000-fold accelerated over background in the presence of the copper(II) complexes in 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) buffer at pH 10.5 and 30 degrees C.
三种双核铜（II）配合物通过 X 射线衍射和紫外/可见分光光度滴定在溶液中进行了表征，并随后评估了它们的糖苷酶样活性。结构分析表明，配合物 2 和 3 的金属间 Cu...Cu 距离（约 3.5Å）相当。尽管如此，配合物在水溶液中的组成却大不相同，这一点通过分光光度滴定得以揭示。在 3-(环己基氨基)-1-丙磺酸（CAPS）缓冲液（pH 值为 10.5 和 30°C）中，在存在铜（II）配合物的情况下，所选硝基苯糖苷的水解速度比背景速度快 11,000 倍。
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