Crowe A J, Hayman M J
Department of Microbiology, State University of New York, Stony Brook 11794-8621.
J Virol. 1991 May;65(5):2533-8. doi: 10.1128/JVI.65.5.2533-2538.1991.
The transforming gene product of the S13 avian erythroblastosis virus, v-sea, is a member of the growth factor receptor class of tyrosine kinases. In the virus genome, the sea sequences are fused in frame to the virus env gene, thereby generating an abnormally large envelope protein because of the presence of a cytoplasmic tyrosine kinase domain. To determine what role these envelope sequences play in v-sea transformation, we generated a myristylated form of v-sea which contains no envelope sequences. In this report, we show that this myristylated sea-encoded protein retained the ability to transform chicken embryo fibroblasts, indicating that envelope sequences are not essential for transformation by the v-sea tyrosine kinase.
S13禽成红细胞增多症病毒的转化基因产物v-sea是酪氨酸激酶类生长因子受体的成员。在病毒基因组中，sea序列与病毒env基因读框融合，由于存在细胞质酪氨酸激酶结构域，从而产生异常大的包膜蛋白。为了确定这些包膜序列在v-sea转化中所起的作用，我们构建了一种不含包膜序列的肉豆蔻酰化形式的v-sea。在本报告中，我们表明这种肉豆蔻酰化的sea编码蛋白保留了转化鸡胚成纤维细胞的能力，这表明包膜序列对于v-sea酪氨酸激酶的转化并非必不可少。
