Smith C M, Henderson J F, Baer H P
J Cyclic Nucleotide Res. 1977 Oct;3(5):347-54.
Experiments were conducted to test the hypothesis that intracellular concentrations of guanosine triphosphate (GTP) regulate the activity and hormonal sensitivity of adenylate cyclase in intact cells. By appropriate treatments, the GTP concentrations of Ehrlich ascites tumor cells could be varied between 28% and 680% of control values. Cyclic AMP concentrations were measured before and after addition of epinephrine in cells containing this range of GTP concentratins. Basal cyclic AMP concentrations were unaffected by changes in GTP concentrations. In cells containing lowered concentrations of GTP, the cyclic AMP concentration following addition of epinephrine was half that in control cells. Elevation of GTP concentrations above normal and had no effect on cyclic AMP concentrations following epinephrine treatment.
鸟苷三磷酸（GTP）的细胞内浓度调节完整细胞中腺苷酸环化酶的活性和激素敏感性。通过适当处理，艾氏腹水瘤细胞的GTP浓度可在对照值的28%至680%之间变化。在含有此GTP浓度范围的细胞中，添加肾上腺素前后测量环磷酸腺苷（cAMP）浓度。基础cAMP浓度不受GTP浓度变化的影响。在GTP浓度降低的细胞中，添加肾上腺素后的cAMP浓度是对照细胞中的一半。将GTP浓度提高到正常水平以上，对肾上腺素处理后的cAMP浓度没有影响。
