Enomoto K, Gill D M
J Supramol Struct. 1979;10(1):51-60. doi: 10.1002/jss.400100106.
The activation of adenylate cyclase in lysed pigeon erythrocytes requires, among several cofactors, a nucleotide which may be ATP, GTP, or many other triphosphates. However, after removal of endogenous nucleotides by gel filtration or by adsorption onto charcoal the requirement can be met only by GTP, or an analog of GTP. The GTP is required during the activation of the cyclase by toxin even if GTP is also included during the subsequent adenylate cyclase assay, conducted without toxin. In the presence of GTP it is possible to assay for the cytosolic protein that is also required for the action of cholera toxin. By gel filtration, its apparent molecular weight is 15,000--20,000.
在溶解的鸽红细胞中，腺苷酸环化酶的激活除了需要几种辅助因子外，还需要一种核苷酸，它可能是ATP、GTP或许多其他三磷酸酯。然而，通过凝胶过滤或吸附到活性炭上去除内源性核苷酸后，只有GTP或GTP类似物才能满足这种需求。即使在随后不使用毒素进行的腺苷酸环化酶测定过程中也加入了GTP，但在毒素激活环化酶的过程中仍需要GTP。在有GTP存在的情况下，可以检测霍乱毒素作用所需的胞质蛋白。通过凝胶过滤，其表观分子量为15,000 - 20,000。
