Demir T, Turgut B, Ozercan I, Gul F C, Ilhan N, Celiker U
Department of Ophthalmology, Firat University School of Medicine, Elazig, Turkey.
Clin Ophthalmol. 2010 Feb 2;4:21-6. doi: 10.2147/opth.s8409.
Trimetazidine (TMZ) has been used to protect against ischemia/reperfusion (I/R) injury of many tissues. We aimed to evaluate the effect of TMZ during retinal I/R in a guinea pig model.
STUDY DESIGN/PATIENTS AND METHODS: An experimental study in retinal I/R. Three groups of five guinea pigs were studied to include a control, placebo, and drug test groups. Prior to the application of 90 minutes of high intraocular pressure (IOP) to induce retinal ischemia followed by 24 hours of reperfusion, we applied intraperitoneal saline to the placebo group and 3 mg/kg of TMZ for the drug test group and repeated the injections at intervals of six hours for four cycles. Both eyes of the animals were enucleated at the end of the reperfusion period. Biochemical assay and histopathologic evaluation was performed on one randomly selected eye of each animal. The level of retinal-free malondialdehyde (MDA) and retinal layer thicknesses were determined and comparisons were then made with the control group.
The mean free MDA level increased in the placebo group (P = 0.006) but not in the drug group (P > 0.05). We observed polymorphonucleated leukocyte infiltration, retinal edema and hydropic degeneration in the retina of the placebo group. However, significant histopathologic change was not observed in specimens of the drug group.
This study suggests TMZ has a beneficial effect on retinal lipid peroxidation and histopathologic changes due to I/R injury.
曲美他嗪（TMZ）已被用于预防多种组织的缺血/再灌注（I/R）损伤。我们旨在评估TMZ在豚鼠视网膜I/R模型中的作用。
研究设计/患者与方法：一项关于视网膜I/R的实验研究。对三组豚鼠进行研究，每组五只，包括对照组、安慰剂组和药物试验组。在施加90分钟高眼压以诱导视网膜缺血，随后进行24小时再灌注之前，我们对安慰剂组腹腔注射生理盐水，对药物试验组腹腔注射3mg/kg的TMZ，并每隔6小时重复注射一次，共进行四个周期。在再灌注期结束时摘除动物的双眼。对每只动物随机选取的一只眼睛进行生化分析和组织病理学评估。测定视网膜游离丙二醛（MDA）水平和视网膜层厚度，然后与对照组进行比较。
安慰剂组平均游离MDA水平升高（P = 0.006），而药物组未升高（P > 0.05）。我们在安慰剂组视网膜中观察到多形核白细胞浸润、视网膜水肿和水样变性。然而，在药物组标本中未观察到明显的组织病理学变化。
本研究表明，TMZ对视网膜脂质过氧化和I/R损伤引起的组织病理学变化具有有益作用。
