Ozden A, Aybek Z, Saydam N, Calli N, Saydam O, Düzcan E, Güner G
Department of Surgery, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Eur Surg Res. 1998;30(4):227-34. doi: 10.1159/000008581.
In this experimental study, we evaluated the effect of trimetazidine (TMZ) on renal ischemia-reperfusion (IR) injury in Sprague-Dawley rats. Renal IR was achieved by a 75-min clamping of the left renal pedicle and subsequent 24 h reperfusion, after right nephrectomy was performed. The rats were randomly divided into three groups: group 1 (sham-operated: no IR injury), group 2 (ischemic control: saline treatment), and group 3 (3 mg/kg TMZ before ischemia). After 24 h of reperfusion, blood samples and renal tissue samples were taken to measure the levels of creatinine, tissue malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) activity. Histopathological changes were evaluated. In addition, the 7-day survival rates in each group were evaluated. We found significant increases in the levels of creatinine and tissue MDA, severe acute tubular necrosis, and a significant decrease in the activity of the GSH-Px in group 2. There were significant decreases in the levels of creatinine and tissue MDA, mild acute tubular necrosis, and a significant increase in activity of the GSH-Px in group 3 when compared with the control group (p <0.05). Statistically significant differences (p <0.05) in survival were noted between the ischemic control and sham-operated and TMZ groups. We have concluded that TMZ is able to protect the kidney from warm IR injury.
在本实验研究中，我们评估了曲美他嗪（TMZ）对Sprague-Dawley大鼠肾缺血再灌注（IR）损伤的影响。在切除右侧肾脏后，通过夹闭左肾蒂75分钟并随后再灌注24小时来实现肾IR。将大鼠随机分为三组：第1组（假手术组：无IR损伤）、第2组（缺血对照组：生理盐水处理）和第3组（缺血前给予3mg/kg TMZ）。再灌注24小时后，采集血样和肾组织样本，以测量肌酐水平、组织丙二醛（MDA）水平和谷胱甘肽过氧化物酶（GSH-Px）活性。评估组织病理学变化。此外，评估每组的7天生存率。我们发现，第2组的肌酐水平和组织MDA水平显著升高，出现严重急性肾小管坏死，GSH-Px活性显著降低。与对照组相比，第3组的肌酐水平和组织MDA水平显著降低，出现轻度急性肾小管坏死，GSH-Px活性显著升高（p<0.05）。缺血对照组与假手术组和TMZ组之间在生存率方面存在统计学显著差异（p<0.05）。我们得出结论，TMZ能够保护肾脏免受热IR损伤。
