HNO Klinik, ZLF 411, University of Basel, Hebelstr 20, 4031 Basel, Switzerland.
Neural Plast. 2009;2009:852492. doi: 10.1155/2009/852492. Epub 2010 Feb 14.
Neural transplantation has emerged as an attractive strategy for the replacement of neurons that have been lost in the central nervous system. Multipotent neural progenitor cells are potentially useful as donor cells to repopulate the degenerated regions. One important aspect of a transplantation strategy is whether transplanted cells are capable of fiber outgrowth with the aim of rebuilding axonal connections within the host brain. To address this issue, we expanded neuronal progenitor from the cortex of embryonic day 15 ubiquitously green fluorescent protein-expressing transgenic mice as neurospheres in vitro and grafted them into the entorhinal cortex of 8-week-old mice immediately after a perforant pathway lesion. After transplantation into a host brain with a lesion of the entorhino-hippocampal projection, the neurosphere-derived cells extended long fiber projections directed towards the dentate gyrus. Our results indicate that transplantation of neurosphere-derived cells might be a promising strategy to replace lost or damaged axonal projections.
神经移植已成为中枢神经系统中丧失神经元替代的一种有吸引力的策略。多能神经祖细胞可作为供体细胞，用于重新填充退化区域。移植策略的一个重要方面是移植细胞是否能够进行纤维生长，以重建宿主大脑内的轴突连接。为了解决这个问题，我们将来自于胚胎第 15 天泛表达绿色荧光蛋白的转基因小鼠的大脑皮质中的神经元祖细胞作为神经球在体外进行扩增，并在穿通纤维通路损伤后立即将其移植到 8 周龄小鼠的内嗅皮层中。在宿主大脑的内嗅-海马投射损伤后，神经球来源的细胞向齿状回延伸出长的纤维投射。我们的结果表明，神经球来源的细胞移植可能是一种有前途的策略，可以替代丢失或受损的轴突投射。
