Department of Zoology, Unit of Biomonitoring and Management, University of Madras, Guindy Campus, Chennai 600025, India.
Microsc Res Tech. 2010 Oct;73(11):1053-8. doi: 10.1002/jemt.20832.
The aim of this study is to evaluate the antitumor effect of indirubin-3-monoxime and its mode of action in benzo(α)pyrene [B(α)P] induced lung cancer in A/J mice. Light microscopic examination of lung sections of [B(α)P] induced lung cancer mice revealed the presence of adenocarcinoma characterized by extensive proliferation of alveolar epithelium and loss of alveolar spaces. The control lung tissue showed a normal architecture with clear alveolar spaces. Interestingly the indirubin-3-monoxime treated groups showed the reduced adenocarcinoma with appearance of alveolar spaces. Transmission Electron Microscopic (TEM) studies of lung sections of [B(α)P] induced lung cancer mice showed the presence of phaemorphic cells with dense granules and increased mitochondria. The lung sections of mice treated with indirubin-3-monoxime showed the presence of shrunken, fragmented, and condensed nuclei implying apoptosis. The effects were dose dependent and prominent in 10 mg/kg/5 d/week groups suggesting the therapeutic role of indirubin analogue against this deadly human malignancy. Here, our results indicate that indirubin-3-monoxime brings antitumor effect against [B(α)P] induced lung cancer by its apoptotic action in A/J mice.
本研究旨在评估靛玉红-3-单肟的抗肿瘤作用及其在苯并（α）芘（B（α）P）诱导的 A/J 小鼠肺癌中的作用机制。B（α）P 诱导的肺癌小鼠肺组织的光镜检查显示存在腺癌，其特征是肺泡上皮广泛增殖和肺泡空间丧失。对照组肺组织显示正常的肺泡空间结构。有趣的是，靛玉红-3-单肟处理组的腺癌减少，出现肺泡空间。B（α）P 诱导的肺癌小鼠肺组织的透射电子显微镜（TEM）研究显示存在含致密颗粒的变形细胞和增加的线粒体。用靛玉红-3-单肟处理的小鼠肺组织显示出皱缩、破碎和浓缩的核，提示凋亡。这些作用呈剂量依赖性，在 10mg/kg/5d/week 组中更为明显，表明靛玉红类似物对这种致命的人类恶性肿瘤具有治疗作用。在这里，我们的结果表明，靛玉红-3-单肟通过其在 A/J 小鼠中的凋亡作用对 B（α）P 诱导的肺癌产生抗肿瘤作用。
