National Nanotechnology Laboratory of CNR-INFM, Italian Institute of Technology Lecce Unit, University of Salento, ISUFI Lecce, 73100 Italy.
Biomacromolecules. 2010 Mar 8;11(3):820-6. doi: 10.1021/bm9014446.
Halloysite is aluminosilicate clay with hollow tubular structure of 50 nm external diameter and 15 nm diameter lumen. Halloysite biocompatibility study is important for its potential applications in polymer composites, bone implants, controlled drug delivery, and for protective coating (e.g., anticorrosion or antimolding). Halloysite nanotubes were added to different cell cultures for toxicity tests. Its fluorescence functionalization by aminopropyltriethosilane (APTES) and with fluorescently labeled polyelectrolyte layers allowed following halloysite uptake by the cells with confocal laser scanning microscopy (CLSM). Quantitative Trypan blue and MTT measurements performed with two neoplastic cell lines model systems as a function of the nanotubes concentration and incubation time indicate that halloysite exhibits a high level of biocompatibility and very low cytotoxicity, rendering it a good candidate for household materials and medicine. A combination of transmission electron microscopy (TEM), scanning electron microscopy (SEM), and scanning force microscopy (SFM) imaging techniques have been employed to elucidate the structure of halloysite nanotubes.
埃洛石是一种具有中空管状结构的铝硅酸盐粘土，外径为 50nm，内径为 15nm。埃洛石的生物相容性研究对于其在聚合物复合材料、骨植入物、控释药物和保护性涂层（例如，防腐或防霉）中的潜在应用非常重要。埃洛石纳米管已被添加到不同的细胞培养物中进行毒性测试。通过氨丙基三乙氧基硅烷（APTES）对其进行荧光功能化，并与荧光标记的聚电解质层结合，使用共聚焦激光扫描显微镜（CLSM）可以观察到细胞对埃洛石的摄取。使用两种肿瘤细胞系模型系统作为纳米管浓度和孵育时间的函数进行的台盼蓝和 MTT 定量测量表明，埃洛石表现出高水平的生物相容性和极低的细胞毒性，使其成为家庭材料和药物的良好候选材料。已采用透射电子显微镜（TEM）、扫描电子显微镜（SEM）和扫描力显微镜（SFM）成像技术来阐明埃洛石纳米管的结构。
