NNL-Istituto Nanoscienze, CNR Via Arnesano 16 Lecce, 73100, Italy.
Macromol Biosci. 2012 Sep;12(9):1265-71. doi: 10.1002/mabi.201200121. Epub 2012 Aug 8.
Halloysite is natural aluminosilicate clay with hollow tubular structure which allows loading with low soluble drugs using their saturated solutions in organic solvents. Resveratrol, a polyphenol known for having antioxidant and antineoplastic properties, is loaded inside these clay nanotubes lumens. Release time of 48 h is demonstrated. Spectroscopic and ζ-potential measurements are used to study the drug loading/release and for monitoring the nanotube layer-by-layer (LbL) coating with polyelectrolytes for further release control. Resveratrol-loaded clay nanotubes are added to breast cell cultures for toxicity tests. Halloysite functionalization with LbL polyelectrolyte multilayers remarkably decrease nanotube self-toxicity. MTT measurements performed with a neoplastic cell lines model system (MCF-7) as function of the resveratrol-loaded nanotubes concentration and incubation time indicate that drug-loaded halloysite strongly increase of cytotoxicity leading to cell apoptosis.
埃洛石是一种天然的铝硅酸盐粘土，具有中空管状结构，允许用低溶解度的药物在有机溶剂中的饱和溶液进行装载。白藜芦醇是一种具有抗氧化和抗肿瘤特性的多酚，它被装载在这些粘土纳米管的管腔中。证明了 48 小时的释放时间。光谱和 ζ-电位测量用于研究药物的装载/释放，并用于监测纳米管的层层（LbL）用聚电解质涂层，以进一步控制释放。将负载白藜芦醇的粘土纳米管添加到乳腺细胞培养物中进行毒性测试。用 LbL 聚电解质多层对埃洛石进行功能化，显著降低了纳米管的自毒性。用一种肿瘤细胞系模型系统（MCF-7）进行的 MTT 测量，作为负载白藜芦醇的纳米管浓度和孵育时间的函数，表明负载药物的埃洛石强烈增加细胞毒性，导致细胞凋亡。
