Queen Mary, University of London, School of Engineering and Material Science, London, UK.
IET Nanobiotechnol. 2010 Mar;4(1):1-9. doi: 10.1049/iet-nbt.2009.0011.
Bovine serum albumin (BSA) protein adsorption on thin spun films of different metal octakishexylthiophthalocyanine [(C(6)S)(8)PcM, M=Cu, Ni] derivatives is investigated by using three independent spectroscopic measurements namely Raman spectroscopy, ellipsometry and surface plasmon resonance imaging. Thermally induced molecular self-reorganisations in the phthalocyanine films are found to have produced the changes in the surface energy which, in turn, control protein adsorption. The amount of BSA adsorption on [(C(6)S)(8)PcNi] is more limited than that on [(C(6)S)(8)PcCu] and this observation is consistent with the results on the surface wettability obtained from the contact angle measurements. The shift from the plasmonic resonance wavelength because of the BSA adsorption was significantly larger for the heat-treated [(C(6)S)(8)PcCu] than as-deposited film. Similar measurements on the [(C(6)S)(8)PcNi] films showed a limited BSA adsorption. The results of surface plasmon resonance experiments are consistent with those obtained from Raman spectroscopic and ellipsometric techniques.
采用三种独立的光谱测量方法，即拉曼光谱、椭圆偏振和表面等离子体共振成像，研究了不同金属八正十六烷硫代酞菁[(C6S)8PcM，M=Cu、Ni]衍生物的薄旋涂薄膜上的牛血清白蛋白(BSA)蛋白吸附。发现酞菁薄膜中热诱导的分子自组织产生了表面能的变化，进而控制蛋白质的吸附。BSA 在[(C6S)8PcNi]上的吸附量比在[(C6S)8PcCu]上的吸附量更有限，这一观察结果与接触角测量得到的表面润湿性结果一致。由于 BSA 吸附引起的等离子体共振波长的位移对于热处理后的[(C6S)8PcCu]比沉积膜大得多。对[(C6S)8PcNi]薄膜的类似测量显示，BSA 的吸附量有限。表面等离子体共振实验的结果与拉曼光谱和椭圆偏振技术的结果一致。
