Departamento de Biologia e Química, Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Ijuí, Brazil.
J Med Food. 2010 Apr;13(2):439-43. doi: 10.1089/jmf.2009.0046.
Mate (Ilex paraguariensis A. St. Hil.) is a raw material used to prepare popular beverages in South America, whose healthy effects have been pointed out. The leaves and stems of mate contain a complex assemblage of saponins, mainly ursolic acid and oleanolic acid glycosides, and their quantification presents difficulties such as weak cromophorous sites and interference of other compounds. On the other hand, the sugar moieties are a chief character that distinguishes them. We developed and validated a high-performance liquid chromatography method using a refractometer detector for quantifying the major saponins in extractive solution of mate leaves. The validation indicates the suitability of the method, presenting linearity in the concentration range between 102.5 to 854.0 microg/mL to matesaponin 1 and between 103.0 to 515.0 microg/mL to matesaponin 3. The repeatability coefficient of variation was 4.70% and 6.61% for matesaponin 1 and matesaponin 3, respectively. The inter-day coefficient of variation was 6.77% and 7.79% for matesaponin 1 and matesaponin 3, respectively. The analysis of plants from Brazilian States Mato Grosso do Sul and Rio Grande do Sul showed the predominance of matesaponins 1, 2, and 3 in the corresponding extractive solution. The sum of these three saponins ranged between 3 and 10 mg/g for mature leaves in the majority of sampled plants.
马黛树（Ilex paraguariensis A. St. Hil.）是一种用于制备南美洲流行饮料的原料，其健康功效已被指出。马黛树的叶子和茎含有复杂的皂甙混合物，主要为熊果酸和齐墩果酸糖苷，其定量存在弱生色团和其他化合物干扰等困难。另一方面，糖基部分是区分它们的主要特征。我们开发并验证了一种使用折射计检测器的高效液相色谱法，用于定量测定马黛树叶提取物中主要皂甙的含量。验证表明该方法的适用性，在 102.5 至 854.0 μg/mL 至马黛皂甙 1 和 103.0 至 515.0 μg/mL 至马黛皂甙 3 的浓度范围内呈线性关系。马黛皂甙 1 和马黛皂甙 3 的重复性变异系数分别为 4.70%和 6.61%。马黛皂甙 1 和马黛皂甙 3 的日间变异系数分别为 6.77%和 7.79%。对巴西马托格罗索州和南里奥格兰德州植物的分析表明，相应提取物中马黛皂甙 1、2 和 3 占优势。在大多数采样植物的成熟叶片中，这三种皂甙的总和在 3 至 10mg/g 之间。
