Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
J Nat Med. 2022 Jun;76(3):654-669. doi: 10.1007/s11418-022-01614-5. Epub 2022 Mar 15.
The methanol extract from the leaves of Ilex paraguariensis A. St.-Hil. (Aquifoliaceae), popularly known as mate, maté, or yerba maté, inhibits the intracellular triglyceride accumulation in HepG2 cells and suppresses the plasma triglyceride elevation in olive oil-treated mice. Three new triterpene saponins, termed mateosides I (1), II (2), and III (3), were isolated from the extract along with 29 known compounds. The structures of 1-3 were elucidated based on chemical and spectroscopic evidence. Among the isolates, principal saponin constituents, 2 and matesaponins 1 (7) and 2 (9), potently inhibited the triglyceride accumulation in HepG2 cells simultaneously treated with oleic acid and high glucose. In vivo assay of the methanol extract of I. paraguariensis revealed that 7 and 9 showed anti-hyperlipidemic activities in olive oil-treated mice. These results suggested that the saponin constituents of I. paraguariensis could be valuable bioactive marker for the anti-obesogenic activity.
从冬青科植物巴拉圭冬青（Ilex paraguariensis A. St.-Hil.）的叶子中提取的甲醇提取物，俗称马黛茶、马黛或耶巴马黛茶，可抑制 HepG2 细胞内甘油三酯的积累，并抑制橄榄油处理的小鼠血浆甘油三酯的升高。从提取物中分离出三种新的三萜皂苷，分别命名为马黛皂甙 I（1）、II（2）和 III（3），以及 29 种已知化合物。根据化学和光谱证据阐明了 1-3 的结构。在分离出的化合物中，主要皂苷成分 2 和马黛皂苷 1（7）和 2（9），同时抑制油酸和高葡萄糖处理的 HepG2 细胞中甘油三酯的积累。体内实验表明，I. paraguariensis 的甲醇提取物中的 7 和 9 对橄榄油处理的小鼠具有抗高血脂作用。这些结果表明，I. paraguariensis 的皂苷成分可能是抗肥胖活性的有价值的生物活性标志物。
