Instituto Nacional de Producción de Biológicos A.N.L.I.S., Ministerio de Salud, Av. Vélez Sarsfield 563, 1281, Buenos Aires, Argentina.
Toxicon. 2010 Jul;55(8):1415-24. doi: 10.1016/j.toxicon.2010.01.015. Epub 2010 Feb 17.
We report a comparison of toxic and enzymatic activities of Bothrops alternatus venoms collected from specimens of different regions of Argentina with a pool of these same venoms. We determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses, as well as indirect hemolysis, hydrolysis of gelatin and fibrinogen. The electrophoretic pattern of the venoms and that of fibrinogen incubated with each of the venoms was studied by SDS-PAGE. The toxic activities were consistent with those reported for these venoms but with noticeable differences between samples which do not seem related to geographical or climatic characteristics. In some cases the pooled venom showed values close to the individual samples, but it was not representative since at least the half of the venoms (4/9) exhibited very different patterns of toxicity. The potency of some activities in the individual venoms ranged from much higher to much lower than those determined in the pooled venom (0.1 to 3 fold). Not all venoms exhibited thrombin-like activity but all hydrolyzed alpha and beta chains of fibrinogen; however, degradation patterns were not always similar. Variation in activities and toxicities do not seem to be related to geographical or climatic characteristics.
我们报告了来自阿根廷不同地区的标本和这些相同毒液的混合毒液的比较，比较了 Bothrops alternatus 毒液的毒性和酶活性。我们测定了致死、出血和促凝（血浆和纤维蛋白原）剂量，以及间接溶血、明胶和纤维蛋白原的水解作用。用 SDS-PAGE 研究了毒液和与每种毒液孵育的纤维蛋白原的电泳模式。毒性活性与这些毒液的报道一致，但样本之间存在明显差异，这些差异似乎与地理或气候特征无关。在某些情况下，混合毒液的值接近单个样本，但它不具有代表性，因为至少一半的毒液（4/9）表现出非常不同的毒性模式。一些毒液的某些活性的效力范围比混合毒液中的测定值高得多（0.1 到 3 倍）或低得多。并非所有毒液都表现出类凝血酶活性，但都水解纤维蛋白原的α和β链；然而，降解模式并不总是相似。活性和毒性的变化似乎与地理或气候特征无关。
