Pharmascience, 6111 Royalmount Ave, Montreal, Quebec H4P 2T4, Canada.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 15;878(9-10):831-5. doi: 10.1016/j.jchromb.2010.01.025. Epub 2010 Jan 25.
A simple and reproducible high performance liquid chromatography-tandem mass spectrometric method was developed for methocarbamol analysis in human plasma. Methocarbamol and the internal standard (IS) were extracted by a protein precipitation method. Under isocratic separation condition the chromatographic run time was 3.0 min. The calibration curve was linear over a range of 150-12,000 ng/mL with good intraday assay and interday assay precision (CV%<10.9%). The method was proven to be sensitive and selective for the analysis of methocarbamol in human plasma for bioequivalence study.
建立了一种简单、重现性好的高效液相色谱-串联质谱法，用于人血浆中甲氨蝶呤的分析。甲氨蝶呤和内标（IS）采用蛋白沉淀法提取。在等度分离条件下，色谱运行时间为 3.0 分钟。校准曲线在 150-12000ng/ml 范围内呈线性，日内和日间测定精密度良好（CV%<10.9%）。该方法灵敏、专属性强，可用于人血浆中甲氨蝶呤的生物等效性研究。
