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鉴定和功能分析 MOC1 相互作用蛋白 1。

Identification and functional analysis of the MOC1 interacting protein 1.

机构信息

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Genet Genomics. 2010 Jan;37(1):69-77. doi: 10.1016/S1673-8527(09)60026-6.

DOI:10.1016/S1673-8527(09)60026-6
PMID:20171579
Abstract

Rice tillering is one of the most important agronomic traits that determine grain yields. Our previous study has demonstrated that the MONOCULM1 (MOC1) gene is a key component that controls the formation of rice tiller buds. To further elucidate the molecular mechanism of MOC1 involved in the regulation of rice tillering, we performed a yeast-two-hybrid screening to identify MOC1 interacting proteins (MIPs). Here we reported that MIP1 interacted with MOC1 both in vitro and in vivo. The overexpression of MIP1 resulted in enhanced tillering and reduced plant height. In-depth characterization of the context of MIP1 and MOC1 would further our understanding of molecular regulatory mechanisms of rice tillering.

摘要

水稻分蘖是决定谷物产量的最重要农艺性状之一。我们之前的研究表明,单分蘖 1 基因(MOC1)是控制水稻分蘖芽形成的关键组成部分。为了进一步阐明 MOC1 参与调控水稻分蘖的分子机制,我们进行了酵母双杂交筛选,以鉴定 MOC1 的互作蛋白(MIPs)。在这里,我们报告 MIP1 在体外和体内均与 MOC1 相互作用。MIP1 的过表达导致分蘖增加和株高降低。深入研究 MIP1 和 MOC1 的背景将有助于我们理解水稻分蘖的分子调控机制。

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