Jiao Kemeng, Xia Guojun, Zhou Yuan, Zhao Chenyu, Yan Huiyuan, Qi Menglei, Xie Pingfan, Ni Yongjing, Zhao Jingxue, Niu Jishan, Chao Zhaofei, Ren Jiangping, Li Lei
Henan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China.
Genes (Basel). 2024 Dec 22;15(12):1652. doi: 10.3390/genes15121652.
The tiller number is a key agronomic trait for increasing the yield potential of wheat ( L.). A number of quantitative trait loci (QTLs) and key genes controlling tillering have been identified, but the regulatory mechanisms remain unclear.
In this study, we utilized the dwarf-monoculm mutant () obtained from the ethyl methane sulfonate (EMS)-treated wheat cultivar Guomai 301. The F populations were constructed using the mutant crossed to multiple tiller parents. The F populations were surveyed for tillering traits at the critical fertility stage for genetic analyses. The extreme-tillering-phenotype plants from the F population were used to construct mixing pools that were analyzed by a wheat 55K SNP array. The tillering genes of were mapped using the wheat 55K SNP array combined with transcriptomic data.
The results showed that the genetic phenotype of is controlled by two dominant genes. The tillering genes of were mapped on the 60-100 Mb region of chromosome 5B and the 135-160 Mb region of chromosome 7A. A total of sixteen candidate genes associated with the tillering trait of were identified. Two candidate genes, TraesCS5B02G058800 and TraesCS7A02G184200, were predicted to be involved in indole acetic acid (IAA) response and transport, which were considered as potential regulatory genes.
This study elucidated the genetic basis of the mutant and provided two valuable reference genes for studying the development and regulatory mechanisms of wheat tillering.
分蘖数是提高小麦产量潜力的关键农艺性状。已经鉴定出许多控制分蘖的数量性状位点（QTL）和关键基因，但调控机制仍不清楚。
在本研究中，我们利用从甲基磺酸乙酯（EMS）处理的小麦品种国麦301中获得的矮秆单茎突变体（）。使用该突变体与多分蘖亲本杂交构建F群体。在关键生育期对F群体的分蘖性状进行调查以进行遗传分析。来自F群体的极端分蘖表型植株用于构建混合池，通过小麦55K SNP芯片进行分析。利用小麦55K SNP芯片结合转录组数据对的分蘖基因进行定位。
结果表明，的遗传表型受两个显性基因控制。的分蘖基因定位在5B染色体的60 - 100 Mb区域和7A染色体的135 - 160 Mb区域。共鉴定出16个与分蘖性状相关的候选基因。两个候选基因TraesCS5B02G058800和TraesCS7A02G184200被预测参与吲哚乙酸（IAA）响应和运输，被认为是潜在的调控基因。
本研究阐明了突变体的遗传基础，为研究小麦分蘖的发育和调控机制提供了两个有价值的参考基因。
