Centre for Human Reproduction Prof Franco Jr, Ribeirão Preto, Sao Paulo, Brazil.
Eur J Obstet Gynecol Reprod Biol. 2010 May;150(1):42-6. doi: 10.1016/j.ejogrb.2010.01.004. Epub 2010 Feb 19.
To evaluate whether intracytoplasmic morphologically selected sperm injection (IMSI) could influence early paternal effects by observing embryo quality at day 2.
The study included 30 couples with at least one of the following criteria: male factor infertility, at least 2 previous failures of implantation or previous miscarriages after IVF/ICSI. Sibling oocytes of each patient were randomly assigned to either the ICSI group or the IMSI group. For IMSI, spermatozoa were selected at 8400x magnification through an inverted microscope equipped with Nomarski differential interference contrast optics, Uplan Apo 100x oil/1.35 objective lens and variable zoom lens. For conventional ICSI, spermatozoa were selected at 400x magnification. An embryo was defined as top quality if there were four identical blastomeres on day 2 with no fragments or multinucleation of blastomeres. Data were analysed using the Wilcoxon and chi-squared tests. The significance level was set at P<0.05. The variables were analysed in relation to the general population and the subpopulations with or without male factor.
A total of 331 MII oocytes (30 oocyte retrievals) were selected and injected by the ICSI (n: 172) or IMSI (n: 159) procedure. For IMSI, only spermatozoa classified as morphologically normal at high magnification were used. No differences (P>0.05) in fertilisation rate (ICSI: 70.9%; IMSI: 70.4%), early embryo cleavage rate (ICSI: 66.9%; IMSI: 60.4%) or cleavage rate (ICSI: 99.2%; IMSI: 99.1%) were observed. On day 2, as compared to ICSI, IMSI provided a similar proportion of top quality embryos (ICSI: 57.8%; IMSI: 52.2%; P>0.05). These results were not influenced by the presence or absence of male factor.
In terms of embryo quality at day 2, IMSI had the same performance as conventional ICSI. However, we cannot exclude the possibility that IMSI effects occur only as a positive later paternal effect.
通过观察第 2 天胚胎质量，评估胞浆内形态选择精子注射（IMSI）是否会影响早期父系效应。
该研究纳入了 30 对夫妇，他们至少符合以下标准之一：男性因素不孕、至少 2 次着床失败或 IVF/ICSI 后流产。每位患者的同胞卵母细胞被随机分配到 ICSI 组或 IMSI 组。对于 IMSI，通过配备诺马斯基微分干涉对比光学、Uplan Apo 100x 油/1.35 物镜和可变缩放透镜的倒置显微镜，在 8400x 放大倍数下选择精子。对于常规 ICSI，在 400x 放大倍数下选择精子。如果第 2 天有 4 个相同的卵裂球，没有碎片或卵裂球多核化，则胚胎被定义为优质胚胎。使用 Wilcoxon 和卡方检验分析数据。显著性水平设为 P<0.05。分析了与一般人群以及有无男性因素的亚人群相关的变量。
共选择和注射了 331 个 MII 卵母细胞（30 个卵母细胞采集），采用 ICSI（n：172）或 IMSI（n：159）程序。对于 IMSI，仅使用高倍镜下形态正常的精子。受精率（ICSI：70.9%；IMSI：70.4%）、早期胚胎卵裂率（ICSI：66.9%；IMSI：60.4%）或卵裂率（ICSI：99.2%；IMSI：99.1%）无差异（P>0.05）。第 2 天，与 ICSI 相比，IMSI 提供了相似比例的优质胚胎（ICSI：57.8%；IMSI：52.2%；P>0.05）。这些结果不受男性因素的存在与否的影响。
就第 2 天的胚胎质量而言，IMSI 的表现与常规 ICSI 相同。然而，我们不能排除 IMSI 效应仅作为一种积极的后期父系效应发生的可能性。
