• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

真核生物翻译起始因子4E的磷酸化在Src转化的细胞系中增加。

Phosphorylation of eukaryotic translation initiation factor 4E is increased in Src-transformed cell lines.

作者信息

Frederickson R M, Montine K S, Sonenberg N

机构信息

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

出版信息

Mol Cell Biol. 1991 May;11(5):2896-900. doi: 10.1128/mcb.11.5.2896-2900.1991.

DOI:10.1128/mcb.11.5.2896-2900.1991
PMID:2017184
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360080/
Abstract

Eukaryotic initiation factor 4F (eIF-4F) is a three-subunit complex that binds the 5' cap structure (m7GpppX, where X is any nucleotide) of eukaryotic mRNAs. This factor facilitates ribosome binding by unwinding the secondary structure in the mRNA 5' noncoding region. The limiting component of the 4F complex is believed to be the 24-kDa cap-binding phosphoprotein, eIF-4E. In this report, we describe the phosphorylation of eIF-4E in response to expression of the tyrosine kinase oncoproteins pp60v-src and pp60c-src527F. The results suggest that eIF-4E functions as a downstream target of the phosphorylation cascade induced by tyrosine-specific protein kinases as well as by effectors of the mitogenic response.

摘要

真核生物起始因子4F（eIF-4F）是一种三聚体复合物，它能结合真核生物mRNA的5'帽结构（m7GpppX，其中X为任意核苷酸）。该因子通过解开mRNA 5'非编码区的二级结构来促进核糖体结合。4F复合物的限速成分被认为是24 kDa的帽结合磷蛋白eIF-4E。在本报告中，我们描述了eIF-4E在酪氨酸激酶癌蛋白pp60v-src和pp60c-src527F表达时的磷酸化情况。结果表明，eIF-4E作为酪氨酸特异性蛋白激酶以及有丝分裂原反应效应物诱导的磷酸化级联反应的下游靶点发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/1a6164bde057/molcellb00139-0575-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/5426f7e0e30b/molcellb00139-0573-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/74a2e7197e24/molcellb00139-0573-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/b536795f5ae3/molcellb00139-0574-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/1221d973eca5/molcellb00139-0575-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/1a6164bde057/molcellb00139-0575-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/5426f7e0e30b/molcellb00139-0573-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/74a2e7197e24/molcellb00139-0573-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/b536795f5ae3/molcellb00139-0574-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/1221d973eca5/molcellb00139-0575-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc5/360080/1a6164bde057/molcellb00139-0575-b.jpg

相似文献

1
Phosphorylation of eukaryotic translation initiation factor 4E is increased in Src-transformed cell lines.真核生物翻译起始因子4E的磷酸化在Src转化的细胞系中增加。
Mol Cell Biol. 1991 May;11(5):2896-900. doi: 10.1128/mcb.11.5.2896-2900.1991.
2
Characterization of wild-type and Ser53 mutant eukaryotic initiation factor 4E overexpression in mammalian cells.野生型和Ser53突变型真核起始因子4E在哺乳动物细胞中过表达的特征分析
J Biol Chem. 1993 Jun 5;268(16):11902-9.
3
Association of initiation factor eIF-4E in a cap binding protein complex (eIF-4F) is critical for and enhances phosphorylation by protein kinase C.起始因子eIF-4E在帽结合蛋白复合物（eIF-4F）中的关联对于蛋白激酶C的磷酸化作用至关重要且能增强该作用。
J Biol Chem. 1990 Jun 25;265(18):10617-21.
4
Ras transformation of cloned rat embryo fibroblasts results in increased rates of protein synthesis and phosphorylation of eukaryotic initiation factor 4E.克隆大鼠胚胎成纤维细胞的Ras转化导致蛋白质合成速率增加以及真核起始因子4E的磷酸化。
J Biol Chem. 1992 May 25;267(15):10659-64.
5
Phorbol esters stimulate phosphorylation of eukaryotic initiation factors 3, 4B, and 4F.佛波酯可刺激真核起始因子3、4B和4F的磷酸化。
J Biol Chem. 1989 Feb 15;264(5):2401-4.
6
Interactions of the eIF-4F subunits in the yeast Saccharomyces cerevisiae.酿酒酵母中真核起始因子4F（eIF-4F）亚基的相互作用。
J Biol Chem. 1992 Oct 15;267(29):21167-71.
7
Eukaryotic initiation factors-4E and -4F stimulate 5' cap-dependent as well as internal initiation of protein synthesis.真核生物起始因子-4E和-4F可刺激5'帽依赖性以及蛋白质合成的内部起始。
J Biol Chem. 1992 Apr 15;267(11):7269-74.
8
Modulation of the mitogenic activity of eukaryotic translation initiation factor-4E by protein kinase C.蛋白激酶C对真核生物翻译起始因子4E促有丝分裂活性的调节
New Biol. 1991 Jun;3(6):601-7.
9
Cap-binding protein (eukaryotic initiation factor 4E) and 4E-inactivating protein BP-1 independently regulate cap-dependent translation.帽结合蛋白（真核生物起始因子4E）和4E失活蛋白BP-1分别独立调节帽依赖性翻译。
Mol Cell Biol. 1996 Oct;16(10):5450-7. doi: 10.1128/MCB.16.10.5450.
10
Multiple mRNAs encode the murine translation initiation factor eIF-4E.多种信使核糖核酸编码小鼠翻译起始因子eIF-4E。
J Biol Chem. 1991 Jun 5;266(16):10446-51.

引用本文的文献

1
Phosphorylation of the mRNA cap-binding protein eIF4E and cancer.mRNA 帽结合蛋白 eIF4E 的磷酸化与癌症。
Cell Signal. 2020 Sep;73:109689. doi: 10.1016/j.cellsig.2020.109689. Epub 2020 Jun 11.
2
SUMO-2 promotes mRNA translation by enhancing interaction between eIF4E and eIF4G.SUMO-2通过增强真核翻译起始因子4E（eIF4E）与真核翻译起始因子4G（eIF4G）之间的相互作用来促进mRNA翻译。
PLoS One. 2014 Jun 27;9(6):e100457. doi: 10.1371/journal.pone.0100457. eCollection 2014.
3
Hypoxia-inducible factor-1α (HIF-1α) promotes cap-dependent translation of selective mRNAs through up-regulating initiation factor eIF4E1 in breast cancer cells under hypoxia conditions.

本文引用的文献

1
Role of mRNA competition in regulating translation: further characterization of mRNA discriminatory initiation factors.mRNA竞争在调节翻译中的作用：mRNA识别起始因子的进一步表征
Proc Natl Acad Sci U S A. 1983 Feb;80(3):663-7. doi: 10.1073/pnas.80.3.663.
2
Preferential stimulation of rabbit alpha globin mRNA translation by a cap-binding protein complex.帽结合蛋白复合物对兔α珠蛋白mRNA翻译的优先刺激作用。
Biochim Biophys Acta. 1984 Nov 22;783(2):122-9. doi: 10.1016/0167-4781(84)90003-4.
3
Differential stimulation of capped mRNA translation in vitro by cap binding protein.
缺氧诱导因子-1α（HIF-1α）通过在缺氧条件下上调起始因子 eIF4E1 促进乳腺癌细胞中选择性 mRNA 的帽依赖性翻译。
J Biol Chem. 2013 Jun 28;288(26):18732-42. doi: 10.1074/jbc.M113.471466. Epub 2013 May 10.
4
RACK1 is a ribosome scaffold protein for β-actin mRNA/ZBP1 complex.RACK1 是核糖体支架蛋白β-肌动蛋白 mRNA/ZBP1 复合物。
PLoS One. 2012;7(4):e35034. doi: 10.1371/journal.pone.0035034. Epub 2012 Apr 16.
5
eIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression.真核生物翻译起始因子4E（eIF4E）磷酸化促进肿瘤发生，并与前列腺癌进展相关。
Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14134-9. doi: 10.1073/pnas.1005320107. Epub 2010 Aug 2.
6
Neurotrophin-3 targets the translational initiation machinery in oligodendrocytes.神经营养因子-3 靶向少突胶质细胞中的翻译起始机制。
Glia. 2009 Dec;57(16):1754-64. doi: 10.1002/glia.20888.
7
A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay.真核生物翻译起始因子4E结合蛋白4E-T在P小体形成和mRNA降解中的作用。
J Cell Biol. 2005 Sep 12;170(6):913-24. doi: 10.1083/jcb.200504039.
8
Active Src elevates the expression of beta-catenin by enhancement of cap-dependent translation.活化的Src通过增强帽依赖性翻译来提高β-连环蛋白的表达。
Mol Cell Biol. 2005 Jun;25(12):5031-9. doi: 10.1128/MCB.25.12.5031-5039.2005.
9
Translation initiation and its deregulation during tumorigenesis.肿瘤发生过程中的翻译起始及其失调。
Br J Cancer. 2002 Apr 8;86(7):1023-7. doi: 10.1038/sj.bjc.6600222.
10
Phosphorylation of eukaryotic translation initiation factor 4E is critical for growth.真核生物翻译起始因子4E的磷酸化对细胞生长至关重要。
Mol Cell Biol. 2002 Mar;22(6):1656-63. doi: 10.1128/MCB.22.6.1656-1663.2002.
帽结合蛋白对体外加帽mRNA翻译的差异刺激作用。
Nature. 1980 May 29;285(5763):331-3. doi: 10.1038/285331a0.
4
The regulation of initiation of mammalian protein synthesis.哺乳动物蛋白质合成起始的调控。
Prog Nucleic Acid Res Mol Biol. 1981;25:127-85. doi: 10.1016/s0079-6603(08)60484-5.
5
Regulation of a ribosomal protein S6 kinase activity by the Rous sarcoma virus transforming protein, serum, or phorbol ester.劳氏肉瘤病毒转化蛋白、血清或佛波酯对核糖体蛋白S6激酶活性的调节。
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7621-5. doi: 10.1073/pnas.82.22.7621.
6
Immunological detection of the messenger RNA cap-binding protein.信使核糖核酸帽结合蛋白的免疫学检测
J Biol Chem. 1985 Jul 5;260(13):7843-9.
7
Regulated phosphorylation and low abundance of HeLa cell initiation factor eIF-4F suggest a role in translational control. Heat shock effects on eIF-4F.HeLa细胞起始因子eIF-4F的磷酸化调控及低丰度表明其在翻译控制中发挥作用。热休克对eIF-4F的影响。
J Biol Chem. 1987 Jan 5;262(1):380-8.
8
High-level synthesis in Escherichia coli of functional cap-binding eukaryotic initiation factor eIF-4E and affinity purification using a simplified cap-analog resin.在大肠杆菌中高效合成功能性帽结合真核起始因子eIF-4E，并使用简化的帽类似物树脂进行亲和纯化。
Gene. 1988 Dec 30;74(2):517-25. doi: 10.1016/0378-1119(88)90184-9.
9
Cap recognition and the entry of mRNA into the protein synthesis initiation cycle.帽识别以及mRNA进入蛋白质合成起始循环。
Trends Biochem Sci. 1988 Feb;13(2):52-6. doi: 10.1016/0968-0004(88)90028-x.
10
Alterations of the lymphocyte-specific protein tyrosine kinase (p56lck) during T-cell activation.T细胞活化过程中淋巴细胞特异性蛋白酪氨酸激酶（p56lck）的改变。
Mol Cell Biol. 1988 Oct;8(10):4353-61. doi: 10.1128/mcb.8.10.4353-4361.1988.