Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Chungju 361-763, South Korea.
Bioresour Technol. 2010 Jun;101(12):4708-16. doi: 10.1016/j.biortech.2010.01.115. Epub 2010 Feb 19.
We investigated the effects of different concentrations of auxin and auxin/cytokinin combinations, inoculum sizes and Murashige and Skoog (MS) medium dilutions on biomass and accumulation of total phenols and flavonoids in adventitious roots of Hypericum perforatum. The optimized conditions for the shake flask culture growth and secondary metabolites production were 0.1mg/l kinetin with 1mg/l indole-3-butyric acid (IBA), an inoculum density of 6g/l fresh weight (FW) and 1/2MS in the 5-week old cultures. By inoculating 6g/l of adventitious roots into a 3-l balloon type bubble bioreactor (BTBB) containing l/2 MS medium supplemented with 0.1mg/l kinetin with 1mg/l IBA, 104.2g/l adventitious roots were harvested containing 56.47+/-0.22 mg/g dry weight (DW) total phenolics, 35.01+/-1.26 mg/g DW flavonoids, 0.97+/-0.06 mg/g DW chlorogenic acid and 1.389+/-0.80 mg/g DW hypericin. The results of our study contribute to optimization and development of bioreactor technology for adventitious root cultures of H. perforatum for the production of hypericin.
我们研究了不同浓度的生长素和生长素/细胞分裂素组合、接种量和 Murashige 和 Skoog（MS）培养基稀释度对贯叶连翘不定根生物量和总酚类和类黄酮积累的影响。在摇瓶培养生长和次生代谢产物生产的优化条件下，为 0.1mg/l 激动素和 1mg/l 吲哚丁酸（IBA），接种量为 6g/l 鲜重（FW）和 1/2MS 在 5 周龄培养物中。通过将 6g/l 的不定根接种到含有 0.1mg/l 激动素和 1mg/l IBA 的 1/2MS 培养基补充的 3 升气球式泡状生物反应器（BTBB）中，收获了 104.2g/l 的不定根，其中含有 56.47+/-0.22mg/g 干重（DW）总酚类、35.01+/-1.26mg/g DW 类黄酮、0.97+/-0.06mg/g DW 绿原酸和 1.389+/-0.80mg/g DW 金丝桃素。我们的研究结果为贯叶连翘不定根培养的生物反应器技术的优化和发展做出了贡献，以生产金丝桃素。
