Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Centre of Biotechnology for Agriculture Research (CEBAR), Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Molecules. 2022 Jul 25;27(15):4744. doi: 10.3390/molecules27154744.
, also known as 'Lempoyang', possesses various phytomedicinal properties, such as anticancer, antimicrobial, anti-inflammatory, antiulcer, and antioxidant properties. Secondary metabolites possessing such properties i.e., zerumbone and α-humulene, are found dominantly in the plant rhizome. Synergistic effects of plant growth hormones and elicitors on in vitro α-humulene and zerumbone production, and biomass growth, in adventitious root culture (AdRC) of cultivated in a two-stage culture are reported. The culture was induced by supplementation of 1.0 mg/L NAA and 2.0 mg/L IBA (dark), and subsequently maintained in medium supplemented with 1 mg/L NAA and 3 mg/L BAP (16:08 light-dark cycle), yielded the production of zerumbone at 3440 ± 168 µg/g and α-humulene at 3759 ± 798 µg/g. Synergistic elicitation by 400 μM methyl jasmonate (MeJa) and 400 μM salicylic acid (SA) resulted in a 13-fold increase in zerumbone (43,000 ± 200 µg/g), while 400 μM MeJa and 600 μM SA produced a 4.3-fold increase in α-humulene (15,800 ± 5100 µg/g) compared to control.
莪术，又名“Lempoyang”，具有多种植物药特性，如抗癌、抗菌、抗炎、抗溃疡和抗氧化特性。具有这些特性的次生代谢产物，如莪术烯和α-葎草烯，主要存在于植物根茎中。本文报道了在离体根培养（AdRC）中，植物生长激素和诱导剂对 培养物中α-葎草烯和莪术烯的产量以及生物量生长的协同作用。该培养物通过补充 1.0 mg/L NAA 和 2.0 mg/L IBA（黑暗）进行诱导，随后在补充 1 mg/L NAA 和 3 mg/L BAP（16:08 光照-黑暗周期）的培养基中维持，产生的莪术烯产量为 3440 ± 168 µg/g，α-葎草烯产量为 3759 ± 798 µg/g。400 μM 茉莉酸甲酯（MeJa）和 400 μM 水杨酸（SA）的协同诱导导致莪术烯产量增加了 13 倍（43,000 ± 200 µg/g），而 400 μM MeJa 和 600 μM SA 使 α-葎草烯产量增加了 4.3 倍（15,800 ± 5100 µg/g），与对照组相比。
