Department of Orthopaedic Surgery, Pontificia Universidad Catolica de Chile, Marcoleta 352, Santiago, Chile.
J Clin Neurosci. 2010 Apr;17(4):481-5. doi: 10.1016/j.jocn.2009.06.024. Epub 2010 Feb 19.
Mesenchymal stem cells (MSCs) have been isolated from various tissues and expanded in culture. MSCs add osteogenic potential to ceramic scaffolds when used together. A spinal fusion rabbit model was used to evaluate whether a pellet of cultured, autologous bone marrow MSCs (BMSCs) with osteogenic differentiation could increase the fusion rate when co-grafted with an autologous bone graft compared to autograft alone. Thirty rabbits were randomly assigned to two groups. Group 1 received bone autograft alone and Group 2 received bone autograft plus a pellet of cultured and differentiated BMSCs. Group 2 rabbits had a bone marrow puncture, after which the BMSC were cultured and osteoblastic differentiation was induced. BMSC cultures were obtained from 12 of 15 rabbits. The 27 rabbits underwent a bilateral, L4-L5 intertransverse fusion with an autograft and in Group 2 rabbits a pellet of differentiated BMSCs was added to the autograft. In Group 1, the fusion rate was 53% (8 of 15 rabbits) and in Group 2 the fusion rate was 0% (p<0.05). Adding differentiated BMSCs in a pellet without a scaffold not only failed to increase fusion rate, but completely inhibited bony growth.
间充质干细胞（MSCs）已从各种组织中分离出来并在培养中扩增。当与陶瓷支架一起使用时，MSCs 可增加成骨潜能。使用兔脊柱融合模型来评估与单独使用自体移植物相比，在用同种异体骨髓 MSC（BMSC）培养物与成骨分化共移植时，培养的自体 BMSC （BMSC）球是否可以增加融合率。将 30 只兔子随机分为两组。第 1 组仅接受自体骨移植，第 2 组接受自体骨移植加培养和分化的 BMSC 球。第 2 组兔子进行骨髓穿刺，然后培养 BMSC 并诱导成骨细胞分化。从 15 只兔子中的 12 只获得了 BMSC 培养物。27 只兔子进行了双侧 L4-L5 横突间融合，使用自体移植物，在第 2 组兔子中，将分化的 BMSC 球添加到自体移植物中。在第 1 组中，融合率为 53％（15 只兔子中的 8 只），在第 2 组中，融合率为 0％（p<0.05）。不使用支架将分化的 BMSC 球添加到其中不仅未能增加融合率，而且完全抑制了骨生长。
