Department of Cardiology, the 4th affiliated hospital of Harbin Medical University, Harbin, PR China.
Exp Mol Pathol. 2010 Apr;88(2):311-5. doi: 10.1016/j.yexmp.2009.12.002. Epub 2009 Dec 11.
Bone marrow mesenchymal stem cells (BMSCs) could differentiate into cardiomyocytes. In this study, we wished to determine whether we could improve ventricular function in a dilated cardiomyopathy (DCM) model in the rabbit by injecting stem cells directly into the ventricular wall.
Japanese rabbits were treated with doxorubicin to induce dilated cardiomyopathy. BMSCs were isolated from isogenic adult rabbits and expanded ex vivo. DCM-model animals received an injection directly into the ventricular wall with either BMSCs or saline (sham-operated control; a third control group underwent no operation). Heart function in all three groups was evaluated by echocardiography and cardiac catheterization. Hearts were later excised for histological and immunostaining analysis. Left ventricular function was significantly better in BMSC-transplanted animals than in controls. Cellular staining of BrdU and N-cadherin, indicating the presence of bone-marrow-derived cells, was found in BMSC-transplanted animals but not in control and sham-operated animals.
BMSC transplantation can improve left ventricular function in DCM. Direct injection into the ventricular wall is an effective approach for stem cell delivery.
骨髓间充质干细胞（BMSCs）可分化为心肌细胞。本研究旨在通过直接向心室壁注射干细胞，观察是否能改善兔扩张型心肌病（DCM）模型的心室功能。
采用阿霉素处理日本大耳白兔诱导扩张型心肌病。从同基因成年兔中分离和体外扩增 BMSCs。DCM 模型动物通过直接向心室壁注射 BMSCs 或生理盐水（假手术对照；第三组对照组不进行任何操作）。通过超声心动图和心导管术评估三组动物的心脏功能。随后取出心脏进行组织学和免疫染色分析。与对照组相比，BMSC 移植组的左心室功能明显改善。BMSC 移植组存在 BrdU 和 N-钙黏蛋白的细胞染色，表明存在骨髓来源的细胞，而对照组和假手术组则没有。
BMSC 移植可改善 DCM 患者的左心室功能。直接向心室壁注射是干细胞递送的有效方法。
