Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Curr Opin Microbiol. 2010 Apr;13(2):198-203. doi: 10.1016/j.mib.2010.01.014. Epub 2010 Feb 18.
This review concerns how Escherichia coli detects environmental inorganic orthophosphate (P(i)) to regulate genes of the phosphate (Pho) regulon by the PhoR/PhoB two-component system (TCS). P(i) control by the PhoR/PhoB TCS is a paradigm of a bacterial signal transduction pathway in which occupancy of a cell surface receptor(s) controls gene expression in the cytoplasm. The P(i) signaling pathway requires seven proteins, all of which probably interact in a membrane-associated signaling complex. Our latest studies show that P(i) signaling involves three distinct processes, which appear to correspond to different states of the sensory histidine kinase PhoR: an inhibition state, an activation state, and a deactivation state. We describe a revised model for P(i) signal transduction of the E. coli Pho regulon.
本综述探讨了大肠杆菌如何通过 PhoR/PhoB 双组分系统（TCS）检测环境无机正磷酸盐（P(i)），以调节磷酸（Pho）调节子的基因。PhoR/PhoB TCS 对 P(i)的控制是细菌信号转导途径的范例，其中细胞表面受体（s）的占据控制细胞质中的基因表达。P(i)信号通路需要七种蛋白质，它们可能都在膜相关信号复合物中相互作用。我们的最新研究表明，P(i)信号涉及三个不同的过程，这似乎对应于感觉组氨酸激酶 PhoR 的不同状态：抑制状态、激活状态和失活状态。我们描述了大肠杆菌 Pho 调节子 P(i)信号转导的修订模型。
